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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration
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Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration

机译：改进的顺序,各向同性和电子glypican-1晶体的密度控制脱水

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摘要

The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time T inc. Of these, the most important was shown to be T inc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.
机译：使用改进的控制脱水的蛋白质晶体衍射质量越来越受欢迎,尽管有仍然相对较少的记录的例子成功。确定控制脱水用于提高晶体的各向异性的人类的核心蛋白蛋白多糖glypican-1。使用盐酸装置脱水。脱水是由谨慎的协议调查的以下参数:脱水率,最终的相对湿度和总孵化时间T公司。重要的是T公司。脱水使用最优协议晶体显示各向异性显著降低和改进的电子密度,允许建设无序的部分结构。

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