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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >GH1-family 6-P-β-glucosidases from human microbiome lactic acid bacteria.
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GH1-family 6-P-β-glucosidases from human microbiome lactic acid bacteria.

机译：GH1-family 6 p -β-glucosidases从人类微生物组乳酸细菌。

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摘要

In lactic acid bacteria and other bacteria, carbohydrate uptake is mostly governed by phosphoenolpyruvate-dependent phosphotransferase systems (PTSs). PTS-dependent translocation through the cell membrane is coupled with phosphorylation of the incoming sugar. After translocation through the bacterial membrane, the β-glycosidic bond in 6'-P-β-glucoside is cleaved, releasing 6-P-β-glucose and the respective aglycon. This reaction is catalyzed by 6-P-β-glucosidases, which belong to two glycoside hydrolase (GH) families: GH1 and GH4. Here, the high-resolution crystal structures of GH1 6-P-β-glucosidases from Lactobacillus plantarum (LpPbg1) and Streptococcus mutans (SmBgl) and their complexes with ligands are reported. Both enzymes show hydrolytic activity towards 6'-P-β-glucosides. The LpPbg1 structure has been determined in an apo form as well as in a complex with phosphate and a glucose molecule corresponding to the aglycon molecule. The S. mutans homolog contains a sulfate ion in the phosphate-dedicated subcavity. SmBgl was also crystallized in the presence of the reaction product 6-P-β-glucose. For a mutated variant of the S.?mutans enzyme (E375Q), the structure of a 6'-P-salicin complex has also been determined. The presence of natural ligands enabled the definition of the structural elements that are responsible for substrate recognition during catalysis.
机译：在乳酸细菌和其他细菌,碳水化合物主要是由吸收phosphoenolpyruvate-dependent磷酸转移酶系统(pts)。再加上通过细胞膜磷酸化传入的糖。通过细菌膜易位,β糖苷键在6 - p -β葡萄糖苷是裂解,释放6 p -β葡萄糖和各自的糖苷配基。6 p -β-glucosidases,属于两个糖苷水解酶(GH)家庭:GH、GH4。gh的高分辨率的晶体结构6 p -β-glucosidases乳杆菌(LpPbg1)和变形链球菌(SmBgl)和他们的报告与配体复合物。酶水解展示活动6 - p -β糖甙。确定在一个apo形式以及在复杂磷酸和葡萄糖分子相应的糖苷配基分子。变形链球菌同族体包含的硫酸盐离子phosphate-dedicated subcavity。结晶反应的存在产品6 p -β葡萄糖。美国吗?6 ' -P-salicin复杂也已确定。天然配体激活的存在结构元素的定义底物识别在负责催化。

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