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首页> 外文期刊>Acta crystallographica. Section D, Biological crystallography. >The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments
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The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

机译：使用迷你-κ测角仪的头大分子晶体学衍射实验

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摘要

Most macromolecular crystallography (MX) diffraction experiments at synchrotrons use a single-axis goniometer. This markedly contrasts with small-molecule crystallography, in which the majority of the diffraction data are collected using multi-axis goniometers. A novel miniaturized κ-goniometer head, the MK3, has been developed to allow macromolecular crystals to be aligned. It is available on the majority of the structural biology beamlines at the ESRF, as well as elsewhere. In addition, the Strategy for the Alignment of Crystals (STAC) software package has been developed to facilitate the use of the MK3 and other similar devices. Use of the MK3 and STAC is streamlined by their incorporation into online analysis tools such as EDNA. The current use of STAC and MK3 on the MX beamlines at the ESRF is discussed. It is shown that the alignment of macromolecular crystals can result in improved diffraction data quality compared with data obtained from randomly aligned crystals.
机译：大多数大分子晶体学(MX)在同步加速器使用衍射实验单轴测角仪。与小分子结晶学,多数的衍射数据收集使用多轴测角仪。小型κ测角仪,MK3,一直发达国家允许大分子晶体一致的。结构生物学beamlines ESRF和其他地方一样。对齐的晶体(STAC)软件包开发促进MK3的使用和其他类似设备。STAC是流线型的并入埃德娜等在线分析工具。使用STAC和MK3的MX beamlines欧洲同步辐射实验室进行了探讨。大分子晶体可能导致改善衍射数据质量与数据从随机获得一致的晶体。

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