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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Hfq binds ribonucleotides in three different RNA-binding sites
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Hfq binds ribonucleotides in three different RNA-binding sites

机译：Hfq结合在三个不同的核苷酸rna结合网站

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摘要

The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface.
机译：Hfq蛋白质形成一个环形的原因homohexamer具有rna结合的活动。有两种截然不同的rna结合的表面位于近端和远端六聚体。绑定的mRNA和小非编码rna (srna),同时为丰富的远端有亲和力RNA序列。各种核苷酸形成复合物Hfq从铜绿假单胞菌测试使用x射线晶体学。位于远端R-site,是a网站保利(A) RNA绑定。在所谓的横向rna结合网站近端表面。远端R-site和近端U-binding网站。三磷酸鸟苷与下Hfq没有形成一个复杂的测试条件。晶体的力量的方法定位核苷酸和预测单链rna结合网站的蛋白质表面。

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