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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Peculiarity in crystal packing of anti-HIV lectin actinohivin in complex with (1-2)mannobiose
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Peculiarity in crystal packing of anti-HIV lectin actinohivin in complex with (1-2)mannobiose

机译：特点在水晶包装抗艾滋病的凝集素在复杂actinohivin mannobiose (1 - 2)

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摘要

Previously, the anti-HIV lectin actinohivin (AH) was cocrystallized with the target (1-2)mannobiose (MB) in the apparent space group P213. However, three MB-bound AH rotamers generated by ±120° rotations around the molecular pseudo-threefold rotation axis are packed randomly in the unit cell according to P212121 symmetry [Hoque et al. (2012). Acta Cryst. D68, 1671-1679]. It was found that the AH used for crystallization contains short peptides attached to the N-terminus [Suzuki et al. (2012). Acta Cryst. F68, 1060-1063], which cause packing disorder. In the present study, the fully mature homogeneous AH has been cocrystallized with MB into two new crystal forms at different pH. X-ray analyses of the two forms reveal that they have peculiar character in that the space groups are the same, P22121, and the unit-cell parameters are almost the same with the exception of the length of the a axis, which is doubled in one form. The use of homogeneous AH resulted in the absence of disorder in both crystals and an improvement in the resolution, thereby establishing the basis for AH binding to the target MB. In addition, the two crystal structures clarify the interaction modes between AH molecules, which is important knowledge for understanding the multiple binding effect generated when two AH molecules are linked together with a short peptide [Takahashi et al. (2011). J. Antibiot. 64, 551-557].
机译：在以前,抗艾滋病凝集素actinohivin(啊)共晶与目标(1 - 2) mannobiose (MB)明显的空间群P213。生成的±120°旋转分子pseudo-threefold旋转轴根据P212121随机在单位细胞1671 - 1679年)。结晶包含短肽n端(铃木et al。(2012)。结晶的。障碍。均匀啊一直在共晶MB成两个新的晶体形式在不同博士x射线分析两种形式的揭示特殊字符的空间组织相同,P22121,晶胞参数几乎是相同的除了吗一个轴的长度,这是翻了一倍的形式。没有晶体和一个障碍从而提高分辨率,建立啊绑定的基础目标MB。此外,这两个晶体结构澄清之间的交互模式啊分子,这是很重要的知识了解多个约束效果时生成两个啊分子是有联系的一起短肽(高桥等。(2011)。

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