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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Various cross-reactivity of the grass pollen group 4 allergens: Crystallographic study of the Bermuda grass isoallergen Cyn d 4
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Various cross-reactivity of the grass pollen group 4 allergens: Crystallographic study of the Bermuda grass isoallergen Cyn d 4

机译：各大草花粉组4过敏原:晶体的研究百慕大草isoallergen Cyn d 4

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摘要

The structure of Cyn d 4, the group 4 allergen from Bermuda grass, is reported at 2.15 ? resolution and is the first crystal structure of a naturally isolated pollen allergen. A conserved N-terminal segment that is only present in the large isoallergens forms extensive interactions with surrounding residues and hence greatly enhances the structural stability of the protein. Cyn d 4 contains an FAD cofactor that is covalently linked to His88 and Cys152. To date, all identified bicovalent flavoproteins are oxidases and their substrates are either sugars or secondary metabolites. A deep large hydrophobic substrate-binding cleft is present. Thus, Cyn d 4 may be an oxidase that is involved in the biosynthesis of a pollen-specific metabolite. Cyn d 4 shares 70% sequence identity with the Pooideae group 4 allergens. Various cross-reactivities between grass pollen group 4 allergens have previously been demonstrated using sera from allergic patients. The protein surface displays an unusually large number of positively charged clusters, reflecting the high pI of 10. 38 decapeptides that cover the solvent-accessible sequences did not show any significant IgE-binding activity using sera with high Cyn d 4 reactivity from four patients, suggesting that the IgE epitopes of Cyn d 4 are predominantly conformational in nature. Several group 4 structures were then modelled and their potential cross-reactive and species-specific IgE epitopes were proposed.
机译：Cyn d的结构4组4过敏原从百慕大草,据报道在2.15 ?分辨率和是第一的晶体结构一个天生孤立的花粉过敏原。只出现在氨基端部分大型isoallergens形式广泛的交互与周边残留,因此极大地提高蛋白质的结构稳定性。Cyn d 4包含一个时尚代数余子式共价链接到His88 Cys152。所有标识bicovalent黄素蛋白糖氧化酶类及其基质或二次代谢物。疏水性substrate-binding裂。因此,Cyn d 4可能是一种氧化酶的生物合成pollen-specific代谢物。与Pooideae组4过敏原。大草花粉组4之间过敏原此前就已被证实使用从过敏患者血清。显示异常多的积极集群,反映出高π的10。溶剂可达38十肽没有任何明显的序列IgE-binding活动用血清高Cyn d 4反应从四个病人,表明Cyn d的IgE抗原表位4主要是构象。结构被模仿和他们的潜力可交叉反应的和特有的IgE抗原表位提出了。

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