Alteration of fluorescent protein spectroscopic properties upon cryoprotection

VonStettenD.; BatotG.O.; Noirclerc-SavoyeM.RoyantA.

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Alteration of fluorescent protein spectroscopic properties upon cryoprotection

机译：荧光蛋白的光谱的改变属性在cryoprotection

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Cryoprotection of a protein crystal by addition of small-molecule compounds may sometimes affect the structure of its active site. The spectroscopic and structural effects of the two cryoprotectants glycerol and ethylene glycol on the cyan fluorescent protein Cerulean were investigated. While glycerol had almost no noticeable effect, ethylene glycol was shown to induce a systematic red shift of the UV-vis absorption and fluorescence emission spectra. Additionally, ethylene glycol molecules were shown to enter the core of the protein, with one of them binding in close vicinity to the chromophore, which provides a sound explanation for the observed spectroscopic changes. These results highlight the need to systematically record spectroscopic data on crystals of light-absorbing proteins and reinforce the notion that fluorescent proteins must not been seen as rigid structures.

机译：Cryoprotection增加的蛋白质晶体有时可能会影响小分子化合物其活性部位的结构。这两种冷冻保护剂和结构的影响甘油和乙二醇在青色荧光蛋白天蓝色。虽然甘油几乎没有明显的效果,乙二醇诱导系统紫外可见吸收红移和荧光发射光谱。乙二醇分子进入蛋白质的核心,其中一个绑定关闭附近的发色团,它提供了良好的解释光谱的变化。需要系统地记录光谱晶体吸光蛋白质和数据强化了这一观念:荧光蛋白质不得被视为刚性结构。

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《Acta crystallographica.Section D. Biological crystallography》 |2012年第11期|1578-1583|共6页
作者
VonStettenD.; BatotG.O.; Noirclerc-SavoyeM.RoyantA.;
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作者单位

CNRS, Institut de Biologie Structurale, Grenoble, France, CEA, Institut de Biologie Structurale;

Structural Biology Group, European Synchrotron Radiation Facility, Grenoble, France;

Structural Biology Group, European Synchrotron Radiation Facility, Grenoble, France, CNRS, Institut;

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正文语种英语
中图分类化学;
关键词
Core protein; Spectrography; glycerolsfluorescent proteinsCryoprotective AgentsRIGID STRUCTURES;

机译：核心蛋白;光谱学;glycerolsfluorescentproteinsCryoprotective AgentsRIGID结构;

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Alteration of fluorescent protein spectroscopic properties upon cryoprotection

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