Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB

PangA.; LiangM.; PrenticeM.B.PickersgillR.W.

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Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB

机译：三聚物的基质频道显示这种乳酸菌细菌microcompartment壳蛋白PduB

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Lactobacillus reuteri metabolizes two similar three-carbon molecules, 1,2-propanediol and glycerol, within closed polyhedral subcellular bacterial organelles called bacterial microcompartments (metabolosomes). The outer shell of the propanediol-utilization (Pdu) metabolosome is composed of hundreds of mainly hexagonal protein complexes made from six types of protein subunits that share similar domain structures. The structure of the bacterial microcompartment protein PduB has a tandem structural repeat within the subunit and assembles into a trimer with pseudo-hexagonal symmetry. This trimeric structure forms sheets in the crystal lattice and is able to fit within a polymeric sheet of the major shell component PduA to assemble a facet of the polyhedron. There are three pores within the trimer and these are formed between the tandem repeats within the subunits. The structure shows that each of these pores contains three glycerol molecules that interact with conserved residues, strongly suggesting that these subunit pores channel glycerol substrate into the metabolosome. In addition to the observation of glycerol occupying the subunit channels, the presence of glycerol on the molecular threefold symmetry axis suggests a role in locking closed the central region.

机译：这种乳酸菌代谢两个相似3个碳分子,2-propanediol和甘油,在封闭的多面亚细胞细菌细胞器称为细菌microcompartments (metabolosomes)。壳牌的propanediol-utilization (Pdu)metabolosome由数以百计的主要组成六角蛋白复合物由六个类型相似的蛋白亚基域结构。microcompartment蛋白质PduB串联结构重复单元内和组装成一个与pseudo-hexagonal三聚物对称。晶格能适合PduA聚合表的主要外壳组件组装多面体的一个方面。三个毛孔内的三聚物,这些都是之间形成的串联重复序列子单元。毛孔包含三个甘油分子与守恒的残留物,强烈表明这些亚基孔通道甘油metabolosome衬底。除了观察甘油占领子单元通道,甘油的存在分子三倍对称轴建议作用在锁封闭的中央地区。

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《Acta crystallographica.Section D. Biological crystallography》 |2012年第12期|1642-1652|共11页
作者
PangA.; LiangM.; PrenticeM.B.PickersgillR.W.;
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作者单位

School of Biological and Chemical Sciences, Queen Mary University of London, 327 Mile End Road;

Department of Microbiology, University College Cork, Cork, Ireland, Alimentary Pharmabiotic Centre;

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正文语种英语
中图分类化学;
关键词
crystal lattices; Lactobacillus rennini; Axis of SymmetryglycerolsEnclosures;

机译：晶体晶格;乳酸菌rennini;轴SymmetryglycerolsEnclosures;

Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB

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