X-ray-excited optical luminescence of protein crystals: A new tool for studying radiation damage during diffraction data collection

OwenR.L.; YorkeB.A.; PearsonA.R.

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X-ray-excited optical luminescence of protein crystals: A new tool for studying radiation damage during diffraction data collection

机译：X-ray-excited光学发光的蛋白质水晶:一个新工具为研究辐射衍射数据收集期间损坏

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During X-ray irradiation protein crystals radiate energy in the form of small amounts of visible light. This is known as X-ray-excited optical luminescence (XEOL). The XEOL of several proteins and their constituent amino acids has been characterized using the microspectrophotometers at the Swiss Light Source and Diamond Light Source. XEOL arises primarily from aromatic amino acids, but the effects of local environment and quenching within a crystal mean that the XEOL spectrum of a crystal is not the simple sum of the spectra of its constituent parts. Upon repeated exposure to X - rays XEOL spectra decay non-uniformly, suggesting that XEOL is sensitive to site-specific radiation damage. However, rates of XEOL decay were found not to correlate to decays in diffracting power, making XEOL of limited use as a metric for radiation damage to protein crystals.

机译：在x射线照射蛋白质晶体辐射能源的形式少量可见光。发光(XEOL)。及其氨基酸组成使用特征显微分光光度计瑞士光源和钻石的光源。酸,但当地环境的影响在一个水晶意味着XEOL淬火光谱的水晶不是简单的总和光谱的组成部分。重复暴露在X -射线XEOL光谱衰减非均匀,表明XEOL是敏感的特定站点辐射损伤。XEOL衰变不关联中被发现在衍射功率衰减,使XEOL有限的使用作为辐射损伤的指标蛋白质晶体。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2012年第5期|505-510|共6页
作者
OwenR.L.; YorkeB.A.; PearsonA.R.;
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作者单位

Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom;

Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, United;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Aromatic Amino Acids; crystals; Radiation InjuriesLIGHT SOURCES;

机译：芳香族氨基酸;晶体;辐射InjuriesLIGHT来源;

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机译：measurement of optical activity (circular birefringence and circular dichroism) for single crystals of cobalt complex and protein crystal by the Extended HaUp method

X-ray-excited optical luminescence of protein crystals: A new tool for studying radiation damage during diffraction data collection

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