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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Nanolitre-scale crystallization using acoustic liquid-transfer technology
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Nanolitre-scale crystallization using acoustic liquid-transfer technology

机译：使用声学Nanolitre-scale结晶液体转移技术

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摘要

Focused acoustic energy allows accurate and precise liquid transfer on scales from picolitre to microlitre volumes. This technology was applied in protein crystallization, successfully transferring a diverse set of proteins as well as hundreds of precipitant solutions from custom and commercial crystallization screens and achieving crystallization in drop volumes as small as 20 nl. Only higher concentrations (>50%) of 2 - methyl-2,4-pentanediol (MPD) appeared to be systematically problematic in delivery. The acoustic technology was implemented in a workflow, successfully reproducing active crystallization systems and leading to the discovery of crystallization conditions for previously uncharacterized proteins. The technology offers compelling advantages in low-nanolitre crystallization trials by providing significant reagent savings and presenting seamless scalability for those crystals that require larger volume optimization experiments using the same vapor-diffusion format.
机译：聚焦声波能量允许准确精确的液体转移picolitre的尺度微升卷。成功应用于蛋白质结晶将一组不同的蛋白质以及从习俗和数以百计的沉淀剂的解决方案商业结晶屏幕和实现结晶在下降20卷一样小问。methyl-2 4-pentanediol (MPD)似乎是系统有问题。声学技术的实现工作流,成功地繁殖活跃结晶系统和导致发现结晶条件以前无特征的蛋白质。技术提供了引人注目的优势通过提供low-nanolitre结晶试验节省试剂和展示无缝的晶体的可伸缩性需要更大的体积优化实验使用相同的扩散格式。

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