A universal indicator dye pH assay for crystallization solutions and other high-throughput applications

NewmanJ.; SayleR.A.; FazioV.J.

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A universal indicator dye pH assay for crystallization solutions and other high-throughput applications

机译：通用指标染料pH值测定结晶的解决方案和其他高通量应用程序

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摘要

In protein crystallization, as well as in many other fields, it is known that the pH at which experiments are performed is often the key factor in the success or failure of the trials. With the trend towards plate-based high-throughput experimental techniques, measuring the pH values of solutions one by one becomes prohibitively time- and reagent-expensive. As part of an HT crystallization facility, a colour-based pH assay that is rapid, uses very little reagent and is suitable for 96-well or higher density plates has been developed.

机译：在蛋白质结晶,以及在许多其他领域一样,众所周知,在pH值实验往往是执行的关键因素成功或失败的试验。趋势plate-based高通量实验技术,测量土壤的pH值一个接一个地变得非常的解决方案时间——和reagent-expensive。结晶设备,colour-based pH值测定快速、试剂,使用很少适合96 -或更高密度板被开发出来。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2012年第8期|1003-1009|共7页
作者
NewmanJ.; SayleR.A.; FazioV.J.;
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作者单位

NextMove Software Ltd, Innovation Centre Unit 23, Science Park, Milton Road, Cambridge CB4 0EY;

Materials Science and Engineering, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
PH; experimentation; Dyesindex testsUniversalCrystallization;

机译：PH;experimentation DyesindextestsUniversalCrystallization;

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A universal indicator dye pH assay for crystallization solutions and other high-throughput applications

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