Construct optimization for studying protein complexes: obtaining diffraction-quality crystals of the pseudosymmetric PSPC1-NONO heterodimer.

Lee M; Passon DM; Hennig SFox AHBond CS

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Construct optimization for studying protein complexes: obtaining diffraction-quality crystals of the pseudosymmetric PSPC1-NONO heterodimer.

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Construct optimization for studying protein complexes: obtaining diffraction-quality crystals of the pseudosymmetric PSPC1-NONO heterodimer.

机译：结构优化为研究蛋白质复合物:获得diffraction-quality晶体《pseudosymmetric PSPC1-NONO heterodimer。

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摘要

The methodology of protein crystallography provides a number of potential bottlenecks. Here, an approach to successful structure solution of a difficult heterodimeric complex of two human proteins, paraspeckle component 1 (PSPC1) and non-POU domain-containing octamer-binding protein (NONO), that are involved in gene regulation and the structural integrity of nuclear bodies termed paraspeckles is described. With the aid of bioinformatic predictions and systematic screening of a panel of constructs, bottlenecks of protein solubility, crystallization, crystal quality and crystallographic pseudosymmetry were overcome in order to produce crystals that ultimately revealed the structure.

机译：蛋白质晶体学的方法提供了许多潜在的瓶颈。一个方法成功的解决方案的结构两人的困难heterodimeric复杂1 (PSPC1)和蛋白质,paraspeckle组件non-POU domain-containing octamer-binding蛋白质(禁忌),参与基因调控和核体称为的结构完整性paraspeckles描述。生物信息学预测和系统筛选的面板结构,瓶颈蛋白质的溶解度,结晶,晶体质量和晶体伪对称克服以产生晶体最终揭示了结构。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2011年第11期|981-987|共7页
作者
Lee M; Passon DM; Hennig SFox AHBond CS;
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作者单位

School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley, WA 6009, Australia.;

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正文语种英语
中图分类化学;
关键词
STRUCTURAL INTEGRITY; Automatic incident detection; POU DomainNR4A2 protein, humancrystalsTraffic BottlenecksmethodologyheterodimerGene regulationpseudosymmetryCrystallization;

机译：结构完整性;自动事件检测;POU DomainNR4A2蛋白质,humancrystalsTrafficBottlenecksmethodologyheterodimerGeneregulationpseudosymmetryCrystallization;

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Construct optimization for studying protein complexes: obtaining diffraction-quality crystals of the pseudosymmetric PSPC1-NONO heterodimer.

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