...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system.
【24h】

Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system.

机译：结构的轻微pseudopilin XcpW从铜绿假单胞菌II型分泌系统。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pseudomonas aeruginosa utilizes the type II secretion machinery to transport virulence factors through the outer membrane into the extracellular space. Five proteins in the type II secretion system share sequence homology with pilin subunits of type IV pili and are called the pseudopilins. The major pseudopilin XcpT(G) assembles into an intraperiplasmic pilus and is thought to act in a piston-like manner to push substrates through an outer membrane secretin. The other four minor pseudopilins, XcpU(H), XcpV(I), XcpW(J) and XcpX(K), play less well defined roles in pseudopilus formation. It was recently discovered that these four minor pseudopilins form a quaternary complex that is presumed to initiate the formation of the pseudopilus and to localize to its tip. Here, the structure of XcpW(J) was refined to 1.85 A resolution. The structure revealed the type IVa pilin fold with an embellished variable antiparallel beta-sheet as also found in the XcpW(J) homologue enterotoxigenic Escherichia coli GspJ(W) and the XcpU(H) homologue Vibrio cholerae EpsU(H). It is proposed that the exposed surface of this sheet may cradle the long N-terminal alpha1 helix of another pseudopilin. The final 31 amino acids of the XcpW(J) structure are instrinsically disordered. Deletion of this unstructured region of XcpW(J) did not prevent type II secretion in vivo.
机译：铜绿假单胞菌利用II型分泌毒性运输机械通过外膜进入的因素细胞外空间。分泌系统共享序列同源性菌毛蛋白亚基的IV型菌毛和被称为pseudopilins。组装成一个intraperiplasmic纤毛和认为在一个活塞式的方式推动通过一个外膜基质分泌素。其他四个小pseudopilins XcpU (H),XcpV(我),XcpW (J)和XcpX (K),少玩定义角色pseudopilus形成。最近发现,这四个小pseudopilins第四纪复杂形式推测发起的形成pseudopilus和本地化的小费。XcpW结构(J)是1.85的精制决议。菌毛蛋白折叠和修饰变量反平行的β褶板还发现的XcpW (J)同系物产肠毒素的大肠杆菌GspJ (W)和XcpU (H)同系物弧菌霍乱弧菌EpsU (H)。这个表可能摇篮表面长另一个pseudopilin的氨基端α螺旋。最后的31个氨基酸XcpW (J)结构instrinsically无序。非结构化的XcpW (J)并没有阻止II型体内分泌。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号