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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain.
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Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain.

机译：disulfide-linked的基于结构的设计低聚物的形式的猴病毒40 (SV40)大T抗原dna结合域。

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摘要

The modular multifunctional protein large T antigen (T-ag) from simian virus 40 orchestrates many of the events needed for replication of the viral double-stranded DNA genome. This protein assembles into single and double hexamers on specific DNA sequences located at the origin of replication. This complicated process begins when the origin-binding domain of large T antigen (T-ag ODB) binds the GAGGC sequences in the central region (site II) of the viral origin of replication. While many of the functions of purified T-ag OBD can be studied in isolation, it is primarily monomeric in solution and cannot assemble into hexamers. To overcome this limitation, the possibility of engineering intermolecular disulfide bonds in the origin-binding domain which could oligomerize in solution was investigated. A recent crystal structure of the wild-type T-ag OBD showed that this domain forms a left-handed spiral in the crystal with six subunits per turn. Therefore, we analyzed the protein interface of this structure and identified two residues that could potentially support an intermolecular disulfide bond if changed to cysteines. SDS-PAGE analysis established that the mutant T-ag OBD formed higher oligomeric products in a redox-dependent manner. In addition, the 1.7 A resolution crystal structure of the engineered disulfide-linked T-ag OBD is reported, which establishes that oligomerization took place in the expected manner.
机译：模块化的多功能蛋白大T抗原(t ag)从猴病毒40协调复制所需的许多事件病毒基因组双链DNA。六聚体组装成单引号和双特定DNA序列位于的起源复制。大T抗原的结合域(t ag ODB)结合GAGGC序列中部地区(网站II)的病毒的起源复制。纯化t ag OBD可以孤立地研究它主要是在溶液中单体的和不能组装成五个一。限制,工程的可能性分子间二硫键结合域可能oligomerize解决方案了。野生型结构t ag OBD显示这一领域形成左手螺旋的每把水晶与六子单元。分析了这种结构的蛋白质界面和确定了两个残留物可能支持一个分子间二硫如果更改为半胱氨酸债券。确定突变t ag OBD形成redox-dependent低聚物的更高的产品的方式。结构工程disulfide-linked t agOBD报告,建立齐聚反应发生在预期的方式。

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