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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection.
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X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection.

机译：高通量X-CHIP:一个集成的平台蛋白质结晶和芯片的x射线衍射数据收集。

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摘要

The X-CHIP (X-ray Crystallization High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The system has been designed for crystallization condition screening, visual crystal inspection, initial X-ray screening and data collection in a high-throughput fashion. X-ray diffraction data acquisition can be performed directly on-the-chip at room temperature using an in situ approach. The capabilities of the chip eliminate the necessity for manual crystal handling and cryoprotection of crystal samples, while allowing data collection from multiple crystals in the same drop. This technology would be especially beneficial for projects with large volumes of data, such as protein-complex studies and fragment-based screening. The platform employs hydrophilic and hydrophobic concentric ring surfaces on a miniature plate transparent to visible light and X-rays to create a well defined and stable microbatch crystallization environment. The results of crystallization and data-collection experiments demonstrate that high-quality well diffracting crystals can be grown and high-resolution diffraction data sets can be collected using this technology. Furthermore, the quality of a single-wavelength anomalous dispersion data set collected with the X-CHIP at room temperature was sufficient to generate interpretable electron-density maps. This technology is highly resource-efficient owing to the use of nanolitre-scale drop volumes. It does not require any modification for most in-house and synchrotron beamline systems and offers a promising opportunity for full automation of the X-ray structure-determination process.
机译：X-CHIP (x射线结晶高通量集成平台)是一种新型芯片结合了多个步骤的的晶体管道结晶衍射数据收集单一设备简化整个过程。系统设计的结晶条件筛选,视觉水晶检验,最初的x射线扫描和数据收集高通量时尚。芯片的直接收购可以执行在室温下使用一个原位方法。芯片的功能消除了必要性和人工晶体处理cryoprotection水晶样品,同时允许从多个晶体的数据收集同样下降。有利于与大量的项目数据,比如蛋白质复合体的研究fragment-based筛选。亲水和疏水同心环表面微型板透明创建一个定义良好的可见光和x线检查和稳定microbatch结晶环境。收集实验证明高质量衍射晶体可以增长和高分辨率的衍射数据集使用这种技术可以收集。此外,单波长的质量反常色散数据集收集的在室温下X-CHIP足以生成可说明的电子密度地图。这种技术资源由于使用nanolitre-scale交易量下降。它不需要任何修改内部和同步加速器beamline系统提供了一个有前途的机会自动化的x射线结构测定的过程。

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