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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Development of high-performance X-ray transparent crystallization plates for in situ protein crystal screening and analysis.
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Development of high-performance X-ray transparent crystallization plates for in situ protein crystal screening and analysis.

机译：开发高性能的x光透明原位结晶板为蛋白质晶体筛选和分析。

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摘要

X-ray transparent crystallization plates based upon a novel drop-pinning technology provide a flexible, simple and inexpensive approach to protein crystallization and screening. The plates consist of open cells sealed top and bottom by thin optically, UV and X-ray transparent films. The plates do not need wells or depressions to contain liquids. Instead, protein drops and reservoir solution are held in place by rings with micrometre dimensions that are patterned onto the bottom film. These rings strongly pin the liquid contact lines, thereby improving drop shape and position uniformity, and thus crystallization reproducibility, and simplifying automated image analysis of drop contents. The same rings effectively pin solutions containing salts, proteins, cryoprotectants, oils, alcohols and detergents. Strong pinning by rings allows the plates to be rotated without liquid mixing to 90 degrees for X-ray data collection or to be inverted for hanging-drop crystallization. The plates have the standard SBS format and are compatible with standard liquid-handling robots.
机译：基于x射线透明结晶板在一本小说drop-pinning技术提供一个灵活、简单和便宜的方法蛋白质结晶筛选。包括开放细胞密封顶部和底部薄的光、紫外线和x光透明的电影。板不需要井或萧条含有液体。储层的解决方案是一个戒指与微米尺寸的在底部的电影。液体接触线,从而提高下降形状和位置一致,因此结晶再现性,简化自动图像分析的内容。包含相同有效环销解决方案盐、蛋白质、冷冻保护剂、油、醇和洗涤剂。旋转的板没有液体混合90度的x射线数据集合或倒悬滴结晶。盘子的SBS格式和标准合成与标准兼容的机器人。

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