...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Decoration of microtubules in solution by the kinesin-14, Ncd.
【24h】

Decoration of microtubules in solution by the kinesin-14, Ncd.

机译：装饰在溶液中微管的

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The kinesin-14, Ncd, is a cellular motor involved in microtubule spindle assembly and contraction during mitosis and meiosis. Like other members of the kinesin superfamily, Ncd consists of two motor heads connected by a linker and a long cargo-carrying stalk. The motor heads hydrolyze ATP to ADP to provide the power stroke that moves them and the cargo along the microtubule. Whereas conventional kinesins move processively along the sense of the microtubule right-handed helix, Ncd moves in the opposite direction, apparently using a different motive mechanism. According to the current model, the microtubule-binding state of Ncd is bound by one head and then released during the motive cycle. This is distinguished from the binding states of conventional kinesins, in which the motor heads are always bound in the motive cycle with alternating one-head and two-head binding. The objective was to determine the extent of binding, the binding states of Ncd in the presence of an ATP analogue, AMPPNP, and whether the binding is cooperative. Small-angle neutron scattering (SANS) of microtubules decorated with a deuterated Ncd construct, Ncd281, in solution containing 42% D(2)O was used. These conditions render the microtubule `invisible' to SANS, while amplifying the SANS from the Ncd constructs. In the presence of AMPPNP, 75% of Ncd281 was not bound. The remainder was bound cooperatively by one of its motor heads to the microtubule.
机译：kinesin-14,非传染性疾病,是一种细胞运动在微管主轴装配和收缩在有丝分裂和减数分裂。驱动蛋白家族,非传染性疾病包括两个汽车头由链接器和长连接货运茎。ATP ADP提供移动的动力冲程他们沿着微管和货物。传统的驱动蛋白进行的在移动意义上的微管右手螺旋,非传染性疾病相反的方向移动,显然使用不同的动机机制。当前模型的microtubule-binding状态非传染性疾病是受一个头,然后释放周期的动机。绑定的传统驱动蛋白,马达头总是绑定的动机周期与交替的一个脑袋和双头绑定。的绑定,绑定状态的非传染性疾病的存在ATP模拟、AMPPNP和绑定是否合作。中子散射(SANS)的微管装饰着一个氘非传染性疾病构造,Ncd281,在溶液中含有42%的D (2) O使用。无“无形的”,而无放大非传染性疾病的结构。AMPPNP, 75%的Ncd281没有绑定。剩余部分由一个绑定合作微管马达头。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号