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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The magic triangle goes MAD: experimental phasing with a bromine derivative.
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The magic triangle goes MAD: experimental phasing with a bromine derivative.

机译：神奇的三角形是疯狂的:实验分阶段与溴的导数。

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摘要

Experimental phasing is an essential technique for the solution of macromolecular structures. Since many heavy-atom ion soaks suffer from nonspecific binding, a novel class of compounds has been developed that combines heavy atoms with functional groups for binding to proteins. The phasing tool 5-amino-2,4,6-tribromoisophthalic acid (B3C) contains three functional groups (two carboxylate groups and one amino group) that interact with proteins via hydrogen bonds. Three Br atoms suitable for anomalous dispersion phasing are arranged in an equilateral triangle and are thus readily identified in the heavy-atom substructure. B3C was incorporated into proteinase K and a multiwavelength anomalous dispersion (MAD) experiment at the Br K edge was successfully carried out. Radiation damage to the bromine-carbon bond was investigated. A comparison with the phasing tool I3C that contains three I atoms for single-wavelength anomalous dispersion (SAD) phasing was also carried out.
机译：实验定相是一个重要的技术大分子结构的解决方案。许多重原子离子浸泡患非特异性绑定,小说类的化合物结合重原子的发达与蛋白质结合的官能团。逐步工具5-amino-2 4 6-tribromoisophthalic酸(B3C)包含三个功能组(两个羧酸盐组和一个氨基)通过氢键相互作用的蛋白质。溴原子适合反常色散逐步被安排在一个等边三角形在重原子,因此很容易识别子结构。蛋白酶K和多波长反常色散(疯狂)实验Br K边缘成功地进行了。bromine-carbon债券了。比较与逐步工具I3C包含三个我为单波长原子反常色散(SAD)定相也执行。

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