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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Progress in rational methods of cryoprotection in macromolecular crystallography.
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Progress in rational methods of cryoprotection in macromolecular crystallography.

机译：理性cryoprotection方法的进展大分子晶体学。

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摘要

Cryogenic cooling of macromolecular crystals is commonly used for X-ray data collection both to reduce crystal damage from radiation and to gather functional information by cryogenically trapping intermediates. However, the cooling process can damage the crystals. Limiting cooling-induced crystal damage often requires cryoprotection strategies, which can involve substantial screening of solution conditions and cooling protocols. Here, recent developments directed towards rational methods for cryoprotection are described. Crystal damage is described in the context of the temperature response of the crystal as a thermodynamic system. As such, the internal and external parts of the crystal typically have different cryoprotection requirements. A key physical parameter, the thermal contraction, of 26 different cryoprotective solutions was measured between 294 and 72 K. The range of contractions was 2-13%, with the more polar cryosolutions contracting less. The potential uses of these results in the development of cryocooling conditions, as well as recent developments in determining minimum cryosolution soaking times, are discussed.
机译：低温冷却的大分子晶体常用的x射线数据集合从辐射和降低晶体损伤通过低温收集功能信息捕获中间体。过程会损坏晶体。cooling-induced晶体损伤通常需要cryoprotection策略,这可能涉及大量的筛选条件和解决方案冷却的协议。针对理性的方法cryoprotection。中描述的环境温度晶体的热力学响应系统。水晶通常有不同的cryoprotection需求。参数,热收缩,26岁不同的防冷冻的测量解决方案在294年和72年之间K。是2 - 13%,与更多的极地cryosolutions吗收缩的更少。cryocooling发展的结果条件,以及最近的进展确定最低cryosolution浸泡时间,进行了讨论。

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