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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Application of protein engineering to enhance crystallizability and improve crystal properties.
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Application of protein engineering to enhance crystallizability and improve crystal properties.

机译：蛋白质工程的应用提高可结晶性和提高晶体的性质。

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摘要

Until recently, protein crystallization has mostly been regarded as a stochastic event over which the investigator has little or no control. With the dramatic technological advances in synchrotron-radiation sources and detectors and the equally impressive progress in crystallographic software, including automated model building and validation, crystallization has increasingly become the rate-limiting step in X-ray diffraction studies of macromolecules. However, with the advent of recombinant methods it has also become possible to engineer target proteins and their complexes for higher propensity to form crystals with desirable X-ray diffraction qualities. As most proteins that are under investigation today are obtained by heterologous overexpression, these techniques hold the promise of becoming routine tools with the potential to transform classical crystallization screening into a more rational high-success-rate approach. This article presents an overview of protein-engineering methods designed to enhance crystallizability and discusses a number of examples of their successful application.
机译：直到最近,蛋白质结晶主要被认为是一个随机事件的调查员有很少或根本没有控制。巨大的技术进步电脑同步源和探测器和同样令人印象深刻的进步晶体的软件,包括自动化模型构建和验证、结晶越来越成为病原反应一步x射线衍射对大分子的研究。然而,随着重组方法它也成为可能的工程师的目标高蛋白质及其复合物倾向于形成晶体的x射线衍射特性。今天在调查中获得的不同的过度,这些技术持有的承诺成为常规的工具将古典的潜力结晶筛选到一个更加合理高成功率的方法。蛋白质工程方法的概述旨在提高可结晶性和讨论的一些例子成功的应用程序。

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