Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.

Villasenor AG; Wong A; Shao AGarg AKuglstatter AHarris SF

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.

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Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.

机译：声矩阵microseeding:改善蛋白质晶体生长的化学偏见。

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A crystal seeding technique is introduced that uses acoustic waves to deliver nanolitre volumes of seed suspension into protein drops. The reduction in delivery volume enables enhanced crystal growth in matrix-seeding experiments without concern for bias from chemical components in the seed-carrying buffer suspension. Using this technique, it was found that while buffer components alone without seed can marginally promote crystal growth in some cases, crystal seeding is far more effective in boosting the number of sparse-matrix conditions that yield protein crystals.

机译：一个水晶播种技术介绍使用声波传递nanolitre卷种子悬挂成蛋白质。减少交货数量允许增强晶体生长在matrix-seeding实验从化学成分没有关心的偏见在seed-carrying缓冲。这种技术,它是发现,虽然缓冲区组件没有种子就略促进晶体生长在某些情况下,晶体在提高播种更有效的稀疏矩阵条件数的收益蛋白质晶体。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2010年第5期|568-576|共9页
作者
Villasenor AG; Wong A; Shao AGarg AKuglstatter AHarris SF;
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作者单位

Department of Discovery Technologies, Roche Palo Alto LLC, 3431 Hillview Avenue, Palo Alto, California 94304, USA.;

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正文语种英语
中图分类化学;
关键词
bias(electronics); crystals; Suspended loadProteinprotein crystal growthStatistical BiasesAcousticsbuffersnanoliterSparse matrixseed inoculationdelivery volumeCrystal growthAcoustic Wave;

机译：偏见(电子);晶体;暂停loadProteinprotein水晶growthStatisticalinoculationdelivery volumeCrystal growthAcoustic波;

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Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.

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