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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >High-resolution X-ray crystal structure of bovine H-protein at 0.88 A resolution.
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High-resolution X-ray crystal structure of bovine H-protein at 0.88 A resolution.

机译：高分辨率x射线晶体结构的牛0.88 h型蛋白在决议。

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摘要

Recent technical improvements in macromolecular X-ray crystallography have significantly improved the resolution limit of protein structures. However, examples of high-resolution structure determination are still limited. In this study, the X-ray crystal structure of bovine H-protein, a component of the glycine cleavage system, was determined at 0.88 A resolution. This is the first ultrahigh-resolution structure of an H-protein. The data were collected using synchrotron radiation. Because of limitations of the hardware, especially the dynamic range of the CCD detector, three data sets (high-, medium- and low-resolution data sets) were measured in order to obtain a complete set of data. To improve the quality of the merged data, the reference data set was optimized for merging and the merged data were assessed by comparing merging statistics and R factors against the final model and the number of visualized H atoms. In addition, the advantages of merging three data sets were evaluated. The omission of low-resolution reflections had an adverse effect on visualization of H atoms in hydrogen-omit maps. Visualization of hydrogen electron density is a good indicator for assessing the quality of high-resolution X-ray diffraction data.
机译：最近的技术进步在大分子x射线晶体学有显著提高蛋白质结构的分辨率极限。然而,高分辨率结构的例子测定仍然是有限的。牛h型蛋白的x射线晶体结构,甘氨酸解理系统的一个组件,0.88决议决定。第一个超高分辨率的结构h型蛋白。同步加速器辐射。硬件,特别是的动态范围CCD探测器,三个数据集(高,中,低分辨率数据集)测量获得一套完整的数据。合并后的数据质量,参考数据设置进行优化合并,合并后的数据通过比较合并统计和评估R因素对最终的模型和数量可视化的H原子。合并三个数据集的优势评估。反射有不利影响可视化的H原子hydrogen-omit地图。可视化的电子密度是氢评估质量的良好指标高分辨率x射线衍射数据。

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