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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >A crystallization screen based on alternative polymeric precipitants.
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A crystallization screen based on alternative polymeric precipitants.

机译：基于替代结晶屏幕聚合物沉淀剂。

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摘要

Most commercially available crystallization screens are sparse-matrix screens with a predominance of inorganic salts and polyethylene glycols (PEGs) as precipitants. It was noted that commercially available screens are largely unsatisfactory for the purpose of the crystallization of multimeric protein and protein-nucleic acid complexes. This was reasoned to be a consequence of the redundancy in screening crystallization parameter space by the predominance of PEG as a precipitant in standard screens and it was suggested that this limitation could be overcome by introducing a variety of other organic polymers. Here, a set of 288 crystallization conditions was devised based on alternative polymeric precipitants and tested against a set of 20 different proteins/complexes; finally, a screen comprising the 96 most promising conditions designed to complement PEG- and salt-based commercial screens was proposed.
机译：大多数商用结晶屏幕与一个稀疏矩阵的屏幕优势的无机盐和聚乙烯乙二醇作为沉淀剂(挂钩)。主要是商用屏幕不满意的目的multimeric蛋白质和结晶protein-nucleic酸复合物。冗余的结果筛选结晶参数空间的优势挂钩作为沉淀剂的标准屏幕,建议这个限制可以克服通过引入多种其他有机聚合物。设计了基于结晶条件选择聚合物沉淀剂和测试根据一组20个不同的蛋白质/情结;最后,一个屏幕组成的96承诺条件旨在补充——挂钩和salt-based商业提出了屏幕。

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