Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes.

Bera AK; Atanasova V; Gamage SRobinson HParsons JF

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Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes.

机译：D-alanylgriseoluteic酸的结构生物合成的蛋白质EhpF,非典型成员退火的腺苷酸酶的总科。

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The structure of EhpF, a 41 kDa protein that functions in the biosynthetic pathway leading to the broad-spectrum antimicrobial compound D-alanylgriseoluteic acid (AGA), is reported. A cluster of approximately 16 genes, including ehpF, located on a 200 kbp plasmid native to certain strains of Pantoea agglomerans encodes the proteins that are required for the conversion of chorismic acid to AGA. Phenazine-1,6-dicarboxylate has been identified as an intermediate in AGA biosynthesis and deletion of ehpF results in accumulation of this compound in vivo. The crystallographic data presented here reveal that EhpF is an atypical member of the acyl-CoA synthase or ANL superfamily of adenylating enzymes. These enzymes typically catalyze two-step reactions involving adenylation of a carboxylate substrate followed by transfer of the substrate from AMP to coenzyme A or another phosphopantetheine. EhpF is distinguished by the absence of the C-terminal domain that is characteristic of enzymes from this family and is involved in phosphopantetheine binding and in the second half of the canonical two-step reaction that is typically observed. Based on the structure of EhpF and a bioinformatic analysis, it is proposed that EhpF and EhpG convert phenazine-1,6-dicarboxylate to 6-formylphenazine-1-carboxylate via an adenylyl intermediate.

机译：EhpF的结构,一个41 kDa蛋白质在生物合成途径导致功能广谱抗菌化合物D-alanylgriseoluteic酸(AGA)报道。大约16个基因的集群,包括ehpF,位于200年kbp质粒原生的某些菌株Pantoea agglomerans编码所需的蛋白质转换AGA的分支酸。Phenazine-1 6-dicarboxylate已被确认作为一个中间AGA生物合成和删除ehpF积累的结果复合体内。这里介绍显示,EhpF是一个典型酰基辅酶合成酶或退火总科的腺苷酸酶。通常涉及催化两步反应腺苷酸羧化物的衬底通过转移衬底的AMP辅酶一个或另一个phosphopantetheine。没有杰出的c端域的特征酶这个家庭,参与phosphopantetheine绑定和下半年的规范化两步反应,通常观察到。基于EhpF和结构生物信息学分析,提出EhpF转铁蛋白小分子EhpG phenazine-1转换和6-dicarboxylate通过腺苷6-formylphenazine-1-carboxylate中间。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2010年第6期|664-672|共9页
作者
Bera AK; Atanasova V; Gamage SRobinson HParsons JF;
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Center for Advanced Research in Biotechnology, The University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA.;

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正文语种英语
中图分类化学;
关键词
Biosynthetic Pathways; Coenzyme A; Chorismic Acidsuperfamilypantetheine phosphateAtypiaPantoea agglomeransCoenzyme A LigasesAdenylation;

机译：生物合成途径、辅酶A、分支agglomeransCoenzyme一LigasesAdenylation;

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Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes.

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