Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme.

Bujacz G; Wrzesniewska B; Bujacz A

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Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme.

机译：Cryoprotection盐的有机的属性酸:一个案例研究的正方晶体凿溶菌酶。

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Currently, the great majority of the data that are used for solving macromolecular structures by X-ray crystallography are collected at cryogenic temperatures. Selection of a suitable cryoprotectant, which ensures crystal stability at low temperatures, is critical for the success of a particular diffraction experiment. The effectiveness of salts of organic acids as potential cryoprotective agents is presented in the following work. Sodium formate, acetate, malonate and citrate were tested, as were sodium potassium tartrate and acetate in the form of potassium and ammonium salts. For each salt investigated, the minimal concentration that was required for successful cryoprotection was determined over the pH range 4.5-9.5. The cryoprotective ability of these organic salts depends upon the number of carboxylic groups; the lowest concentration required for cryoprotection was observed at neutral pH. Case-study experiments conducted using the tetragonal form of hen egg-white lysozyme (HEWL) confirmed that salts of organic acids can successfully act as cryoprotective agents of protein crystals grown from high concentrations of inorganic salts. When crystals are grown from solutions containing a sufficient concentration of organic acid salts no additional cryoprotection is needed as the crystals can safely be frozen directly from the crystallizing buffers.

机译：目前,绝大多数的数据用于解决大分子结构x射线晶体学在低温收集温度。冷冻保护剂,保证晶体的稳定性在低温下,对成功至关重要一个特定的衍射实验。的有机酸盐的有效性潜在的冷冻保存剂提出了下面的工作。丙二酸酯和柠檬酸进行测试,钠醋酸钾酒石酸和的形式钾和铵盐。调查的最小浓度成功的cryoprotection所需pH值在4.5 - -9.5范围决定的。这些有机盐防冷冻的能力取决于数量的羧基组;cryoprotection所需的最低浓度在中性ph案例研究观察吗实验使用正方形式母鸡的蛋清溶菌酶(HEWL)确认有机酸盐可以成功地扮演冷冻剂的蛋白质晶体生长从高浓度的无机盐。从解决方案包含一个晶体生长足够浓度的有机酸盐没有需要额外cryoprotection作为晶体可以安全地直接从冻结结晶缓冲区。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2010年第7期|789-796|共8页
作者
Bujacz G; Wrzesniewska B; Bujacz A;
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作者单位

Institute of Technical Biochemistry, Technical University of Lodz, Poland.;

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正文语种英语
中图分类化学;
关键词
Organic salts; sodium potassium tartrate; ammonium saltsX-ray crystallographyorganic acidSaltsinorganic saltscrystalsCase studiesMuramidaseCryoprotective AgentsAcetatesCitrates;

机译：有机盐、酒石酸钾钠、铵saltsX-ray crystallographyorganicacidSaltsinorganic saltscrystalsCasestudiesMuramidaseCryoprotective;

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Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme.

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