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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection.
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Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection.

机译：衍射制图:应用微光束大分子晶体学样品评估和数据收集。

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摘要

Crystals of biological macromolecules often exhibit considerable inter-crystal and intra-crystal variation in diffraction quality. This requires the evaluation of many samples prior to data collection, a practice that is already widespread in macromolecular crystallography. As structural biologists move towards tackling ever more ambitious projects, new automated methods of sample evaluation will become crucial to the success of many projects, as will the availability of synchrotron-based facilities optimized for high-throughput evaluation of the diffraction characteristics of samples. Here, two examples of the types of advanced sample evaluation that will be required are presented: searching within a sample-containing loop for microcrystals using an X-ray beam of 5 microm diameter and selecting the most ordered regions of relatively large crystals using X-ray beams of 5-50 microm in diameter. A graphical user interface developed to assist with these screening methods is also presented. For the case in which the diffraction quality of a relatively large crystal is probed using a microbeam, the usefulness and implications of mapping diffraction-quality heterogeneity (diffraction cartography) are discussed. The implementation of these techniques in the context of planned upgrades to the ESRF's structural biology beamlines is also presented.
机译：晶体的生物大分子表现出相当大的晶间和intra-crystal衍射质量的变化。这需要很多样品的评价数据收集之前,这种做法已经广泛的高分子晶体学。对解决更加雄心勃勃的项目,新的样本评价将自动化的方法成为许多项目的成功至关重要,就像synchrotron-based的可用性设施优化高通量评估的衍射特性样本。先进的样品评估,还是需要的介绍:内搜索试样中微晶核使用一个循环x射线束5 microm直径和选择大多数命令的区域相对较大的晶体使用x射线束5-50 microm直径。图形用户界面开发的协助这些筛选方法。的情况下衍射的质量相对较大的晶体探测使用微光束的作用和影响映射diffraction-quality异质性(衍射制图)进行了讨论。实现这些技术的背景计划升级到欧洲同步辐射实验室的结构生物学beamlines。

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