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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Inclusion of weak high-resolution X-ray data for improvement of a group II intron structure.
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Inclusion of weak high-resolution X-ray data for improvement of a group II intron structure.

机译：高分辨率的x射线数据疲弱一组II基因内区结构的改进。

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摘要

It is common to report the resolution of a macromolecular structure with the highest resolution shell having an averaged I/sigma(I) > or = 2. Data beyond the resolution thus defined are weak and often poorly measured. The exclusion of these weak data may improve the apparent statistics and also leads to claims of lower resolutions that give some leniency in the acceptable quality of refined models. However, the inclusion of these data can provide additional strong constraints on atomic models during structure refinement and thus help to correct errors in the original models, as has recently been demonstrated for a protein structure. Here, an improved group II intron structure is reported arising from the inclusion of these data, which helped to define more accurate solvent models for density modification during experimental phasing steps. With the improved resolution and accuracy of the experimental phases, extensive revisions were made to the original models such that the correct tertiary interactions of the group II intron that are essential for understanding the chemistry of this ribozyme could be described.
机译：通常报告的决议最高的大分子结构决议壳有平均I /σ(I) >或= 2。是薄弱的,经常测量。这些数据疲弱可能会改善明显统计和也会导致低的说法决议,给一些宽大处理改进模型的可接受的质量。这些数据可以提供额外的强烈限制原子模型在结构优化,从而帮助纠正错误的原始模型,最近被证实为蛋白质结构。结构是报道的包容这些数据,帮助定义更多的准确的溶剂密度模型修改在实验进行的步骤。分辨率和精度的提高实验阶段,广泛的修订这样正确的原始模型三级相互作用组的二世的内含对于理解至关重要的化学这种核糖酶可以被描述。

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