Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: Structural basis for the absence of ATP sulfurylase activity

GayS.C.; SegelI.H.; FisherA.J.

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Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: Structural basis for the absence of ATP sulfurylase activity

机译：结构的两个域hexameric APS激酶从硫杆菌denitrificans:结构基础对ATP硫酸化酶活动的缺失

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The Tbd-0210 gene of the chemolithotrophic bacterium Thiobacillus denitrificans is annotated to encode a 60.5 kDa bifunctional enzyme with ATP sulfurylase and APS kinase activity. This putative bifunctional enzyme was cloned, expressed and structurally characterized. The 2.95 ? resolution X-ray crystal structure reported here revealed a hexa-meric assembly with D 3 symmetry. Each subunit contains a large N-terminal sulfurylase-like domain and a C-terminal APS kinase domain reminiscent of the two-domain fungal ATP sulfurylases of Penicillium chrysogenum and Saccharo-myces cerevisiae, which also exhibit a hexameric assembly. However, the T. denitrificans enzyme exhibits numerous structural and sequence differences in the N-terminal domain that render it inactive with respect to ATP sulfurylase activity. Surprisingly, the C-terminal domain does indeed display APS kinase activity, indicating that this gene product is a true APS kinase. Therefore, these results provide the first structural insights into a unique hexameric APS kinase that contains a nonfunctional ATP sulfurylase-like domain of unknown function.

机译：chemolithotrophic tbd - 0210基因细菌硫杆菌denitrificans注释编码60.5 kDa双功能酶和ATP硫酸化酶和APS激酶活性。假定的双功能酶被克隆,表达和结构特征。2.95 ?这里报道显示hexa-meric组装3 D对称。氨基端sulfurylase-like域和一个c端APS激酶域的两个域真菌ATP硫酸化酶特异chrysogenum和Saccharo-myces酵母也表现出hexameric组装。t . denitrificans酶展品很多结构和序列差异n端结构域,使它不活跃对ATP硫酸化酶的活动。令人惊讶的是,c端域确实显示APS激酶活性,表明这一点基因产物是一个真正的APS激酶。这些结果提供了第一个结构见解独特hexameric APS激酶包含一个非功能性ATP sulfurylase-like未知函数的域。

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《Acta crystallographica.Section D. Biological crystallography》 |2009年第10期|1021-1031|共11页
作者
GayS.C.; SegelI.H.; FisherA.J.;
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作者单位

Section of Molecular and Cellular Biology, University of California, One Shields Avenue, Davis, CA;

Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, United;

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正文语种英语
中图分类化学;
关键词
Thiobacillus denitrificans; Sulfate Adenylyltransferase; adenylylsulfate kinase;

机译：硫酸硫杆菌denitrificans;

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Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: Structural basis for the absence of ATP sulfurylase activity

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