Overproduction, purification and structure determination of human dual-specificity phosphatase 14

LountosG.T.; TropeaJ.E.; CherryS.WaughD.S.

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Overproduction, purification and structure determination of human dual-specificity phosphatase 14

【24h】

Overproduction, purification and structure determination of human dual-specificity phosphatase 14

机译：生产过剩,净化和结构测定人类dual-specificity磷酸酶14

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Dual-specificity phosphatases (DUSPs) are enzymes that participate in the regulation of biological processes such as cell growth, differentiation, transcription and metabolism. A number of DUSPs are able to dephosphorylate phosphoryl-ated serine, threonine and tyrosine residues on mitogen-activated protein kinases (MAPKs) and thus are also classified as MAPK phosphatases (MKPs). As an increasing number of DUSPs are being identified and characterized, there is a growing need to understand their biological activities at the molecular level. There is also significant interest in identifying DUSPs that could be potential targets for drugs that modulate MAPK-dependent signaling and immune responses, which have been implicated in a variety of maladies including cancer, infectious diseases and inflammatory disorders. Here, the overproduction, purification and crystal structure at 1.88 ? resolution of human dual-specificity phosphatase 14, DUSP14 (MKP6), are reported. This structural information should accelerate the study of DUSP14 at the molecular level and may also accelerate the discovery and development of novel therapeutic agents.

机译：Dual-specificity磷酸酶(DUSPs)的酶参与调节的生物过程,如细胞生长、分化、转录和新陈代谢。可以脱去磷酸phosphoryl-ated吗丝氨酸、苏氨酸和酪氨酸残基增殖蛋白激酶(MAPKs)和因此也被归类为MAPK磷酸酶(MKPs)。被识别和特征,有一个越来越需要了解他们的生物在分子水平上的活动。识别DUSPs感兴趣可能是潜在目标药物吗调节MAPK-dependent信号和免疫反应,已涉及多种疾病,包括癌症、传染性疾病和炎症性疾病。生产过剩、净化和晶体结构在1.88吗?DUSP14 dual-specificity磷酸酶14日(MKP6),报告。加速DUSP14在分子的研究水平,也可能加速发现开发新型治疗药物。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2009年第10期|1013-1020|共8页
作者
LountosG.T.; TropeaJ.E.; CherryS.WaughD.S.;
展开▼
作者单位

Macromolecular Crystallography Laboratory, National Cancer Institute at Frederick, PO Box B;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
Drug Liberation; DUSP14 gene; Dual-Specificity PhosphatasesOverproductionMitogen-Activated Protein Kinase Phosphatases;

机译：药物解放;DUSP14基因;Dual-SpecificityPhosphatasesOverproductionMitogen-Activated蛋白激酶磷酸酶;

相似文献

外文文献
中文文献

1. Electrochemical Determination of Alkaline Phosphatase in Human Serum by Differential Pulse Voltammetry [J] . SUN Wei, JIAO Kui, WANG Hai-yu, 高等学校化学研究（英文版） . 2004,第006期
2. Purification and Primary Structure Determination of a Novel Polypeptide Isolated from Mistletoe Viscum coloratum [J] . Jing Lin KONG, Xiu Bao DU, Chong Xu FAN, 中国化学快报（英文版） . 2004,第011期
3. Dual-specificity phosphatase 6 (DUSP6): a review of its molecular characteristics and clinical relevance in cancer [J] . Muhammad Khairi Ahmad, Nur Ainina Abdollah, Nurul Husna Shafie, 癌症生物学与医学（英文版） . 2018,第001期
4. Dual-specificity phosphatase 6（DUSP6）： a review of its molecular characteristics and clinical relevance in cancer [J] . Muhammad Khairi Ahmad, Nur Ainina Abdollah, Nurul Husna Shafie, 癌症生物学与医学：英文版 . 2018,第001期
5. Dual-specificity phosphatase 6(DUSP6): a review of its molecular characteristics and clinical relevance in cancer [J] . Muhammad Khairi Ahmad, Nur Ainina Abdollah, Nurul Husna Shafie, 癌症生物学与医学：英文版 . 2018,第001期
6. Ascariasis in Humans and Pigs on Small-Scale Farms, Maine, USA, 2010–2013 (http://wwwnc.cdc.gov/eid/article/21/2/14-0048) [J] . Kate Colby, Susan E Manning, Donald Hoenig, Emerging Infectious Diseases . 2015,第2期

机译：美国缅因州小规模农场中人和猪的Human虫病，2010-2013年（http://wwwnc.cdc.gov/eid/article/21/2/14-0048）
7. 18th Annual Meeting of the Organization for Human Brain Maooing(2012年6月10~14日，北京) [J] . 北城圭一 Brain and nerve =: Shinkei kenkyu no shinpo . 2013,第3期

机译：18th Annual Meeting of the Organization for Human Brain Maooing(2012年6月10~14日，北京)
8. Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉：I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2 [D] . Yu, Qinhong. 2019

机译：Structure Determination and Mechanistic Insights of: I.Cyanobacteriochrome NpR6012g4 Light Sensor Protein in Phototaxis II.Retinal Degeneration 3 (RD3) Protein in Vision III.Ryanodine Receptor 2 (RyR2) Regulation by Calmodulin (CaM) in Cardiac Function =结构测定和机理洞悉：I.趋光性中的蓝细菌色素NpR6012g4光敏蛋白 II.视觉作用中的视网膜退化蛋白3 III.心脏功能中的钙调蛋白调控兰诺定受体2
9. Presence and distribution of 14-3-3 proteins in human ocular surface tissues [O] . Jwalitha Shankardas, Michelle Senchyna, Slobodan D. Dimitrijevich 2008

机译：human人类眼表组织中14-3-3蛋白的存在与分布
10. Purification of human tissue prokallikrein excreted from insect cells by liquid chromatography [O] . Yang Q, Liang Z, Zhang LH, 2005

机译：purification of human tissue prokallikrein excreted from insect cells by liquid chromatography
11. Anaylsis and Load Testing of Two Steel through Truss Bridges in Indiana. BridgeStructure No. 58-14-3244 and Bridge Structure No. 46-11-1316. (Includes Technical Summary) [R] . Malone, B. J., Heffron, M. A., Ramirez, J. A., 1996

机译：印第安纳州两根钢桁梁桥的分析与荷载试验。 Bridgestructure No. 58-14-3244和Bridge structure No. 46-11-1316。（包括技术摘要）

Overproduction, purification and structure determination of human dual-specificity phosphatase 14

摘要

著录项

相似文献

相关主题

期刊订阅