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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Novel structural features in the GMC family of oxidoreductases revealed by the crystal structure of fungal aryl-alcohol oxidase
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Novel structural features in the GMC family of oxidoreductases revealed by the crystal structure of fungal aryl-alcohol oxidase

机译：小说在GMC家族的结构特点氧化还原酶晶体结构的显示的真菌aryl-alcohol氧化酶

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摘要

Lignin biodegradation, a key step in carbon recycling in land ecosystems, is carried out by white-rot fungi through an H_2O_2-dependent process denned as enzymatic combustion. Pleurotus eryngii is a selective lignin-degrading fungus that produces H_2O_2 during redox cycling of p-anisylic compounds involving the secreted flavoenzyme aryl-alcohol oxidase (AAO). Here, the 2.4 A resolution X-ray crystal structure of this oxidoreductase, which catalyzes dehydrogenation reactions on various primary polyunsaturated alcohols, yielding the corresponding aldehydes, is reported. The AAO crystal structure was solved by single-wavelength anomalous diffraction of a selenomethionine derivative obtained by Escherichia coli expression and in vitro folding. This monomeric enzyme is composed of two domains, the overall folding of which places it into the GMC (glucose-methanol-choline oxidase) oxidoreductase family, and a noncovalently bound FAD cofactor. However, two additional structural elements exist in the surroundings of its active site that modulate the access of substrates; these are absent in the structure of the model GMC oxidoreductase glucose oxidase. The folding of these novel elements gives rise to a funnel-like hydrophobic channel that connects the solvent region to the buried active-site cavity of AAO. This putative active-site cavity is located in front of the re side of the FAD isoalloxazine ring and near two histidines (His502 and His546) that could contribute to alcohol activation as catalytic bases. Moreover, three aromatic side chains from two phenylalanines (Phe397 and Phe502) and one tyrosine (Tyr92) at the inner region of the channel form an aromatic gate that may regulate the access of the enzyme substrates to the active site as well as contribute to the recognition of the alcohols that can effectively be oxidized by AAO.
机译：木质素生物降解,碳的关键一步回收在陆地生态系统,是由通过一个H_2O_2-dependent白腐真菌酶燃烧过程窝。eryngii选择性lignin-degrading真菌期间产生H_2O_2氧化还原循环p-anisylic化合物包括分泌黄素酶aryl-alcohol氧化酶(氧化铝)。分辨率2.4 x射线晶体结构氧化还原酶,催化脱氢反应在各种主要的多不饱和醇,产生相应的醛,据报道。由单波长反常衍射硒代蛋氨酸导数得到大肠杆菌表达和体外折叠。这个单体的酶是由两个域,到整个折叠的地方GMC (glucose-methanol-choline氧化酶)氧化还原酶家族,和共价结合代数余子式的时尚。在活跃的环境元素存在网站,调节基质的访问;这些是缺席的结构模型GMC氧化还原酶葡萄糖氧化酶。这些小说元素产生烟囱似的疏水通道连接溶剂区域埋活性部位腔的氧化铝。位于前面的时尚异咯嗪环和附近两个组氨酸(His502和His546)可能导致酒精激活催化基地。从两三个芳香族侧链苯丙氨酸(Phe397和Phe502)和1酪氨酸(Tyr92)的内部区域通道形成一个芳香可能调节门酶底物的访问活动网站的识别能有效被氧化的醇

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