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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The effect of protein-precipitant interfaces and applied shear on the nucleation and growth of lysozyme crystals
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The effect of protein-precipitant interfaces and applied shear on the nucleation and growth of lysozyme crystals

机译：protein-precipitant接口和的影响应用剪切的成核和增长溶菌酶晶体

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摘要

This paper is concerned with the effect of protein-precipitant interfaces and externally applied shear on the nucleation and growth kinetics of hen egg-white lysozyme crystals. The early stages of microbatch crystallization of lysozyme were explored using both optical and confocal fluorescence microscopy imaging. Initially, an antisolvent (precipitant) was added to a protein drop and the optical development of the protein-precipitant interface was followed with time. In the presence of the water-soluble polymer poly(ethylene glycol) (PEG) a sharp interface was observed to form immediately within the drop, giving an initial clear separation between the lighter protein solution and the heavier precipitant. This interface subsequently became unstable and quickly developed within a few seconds into several unstable 'fingers' that represented regions of high concentration-gradient interfaces. Confocal microscopy demonstrated that the subsequent nucleation of protein crystals occurred preferentially in the region of these interfaces. Additional experiments using an optical shearing system demonstrated that oscillatory shear significantly decreased nucleation rates whilst extending the growth period of the lysozyme crystals. The experimental observations relating to both nucleation and growth have relevance in developing efficient and reliable protocols for general crystallization procedures and the controlled crystallization of single large high-quality protein crystals for use in X-ray crystallography.
机译：本文关注的效果protein-precipitant接口和外部应用剪切成核和生长动力学母鸡蛋清溶菌酶晶体。早期microbatch结晶使用光学和溶菌酶进行了探讨共焦荧光显微镜成像。最初,一个antisolvent(沉淀剂)补充道降低了蛋白质和光学的发展protein-precipitant界面之后随着时间的推移。聚合物聚(乙二醇)(挂钩)一把锋利的观察界面形成内立即下降,初步明确的分离和较轻的蛋白质之间的解决方案重的沉淀剂。变得不稳定和快速发展几秒到几个不稳定的“手指”代表地区的高浓度梯度的接口。显微镜显示,随后蛋白质晶体的成核发生这些接口的地区优先。额外使用光学剪切实验系统显示振荡剪切显著降低成核率的同时延长生长期的溶菌酶晶体。成核和生长都有相关性发展有效的和可靠的协议一般和结晶过程控制结晶的单身大高质量的蛋白质晶体用于x射线晶体学。

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