• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure of laminin-binding adhesin (Lmb) from Streptococcus agalactiae
【24h】

Structure of laminin-binding adhesin (Lmb) from Streptococcus agalactiae

机译：结构laminin-binding adhesin (Lmb)链球菌agalactiae

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Adhesion/invasion of pathogenic bacteria is a critical step in infection and is mediated by surface-exposed proteins termed adhesins. The crystal structure of recombinant Lmb, a laminin-binding adhesin from Streptococcus agalactiae, has been determined at 2.5 ? resolution. Based on sequence and structural homology, Lmb was placed into the cluster 9 family of the ABC (ATP-binding cassette) transport system. The structural organization of Lmb closely resembles that of ABC-type solute-binding proteins (SBPs), in which two structurally related globular domains interact with each other to form a metal-binding cavity at the interface. The bound zinc in Lmb is tetrahedrally coordinated by three histidines and a glutamate from both domains. A comparison of Lmb with other metal transporters revealed an interesting feature of the dimerization of molecules in the crystallographic asymmetric unit in all zinc-binding transporters. A closer comparison of Lmb with the zinc-binding ZnuA from Escherichia coli and Synechocystis 6803 suggested that Lmb might undergo a unique structural rearrangement upon metal binding and release. The crystal structure of Lmb provides an impetus for further investigations into the molecular basis of laminin binding by human pathogens. Being ubiquitous in all serotypes of group B streptococcus (GBS), the structure of Lmb may direct the development of an efficient vaccine.
机译：附着力/致病菌的入侵感染和关键的一步是由surface-exposed蛋白质称为丰富。重组Lmb的晶体结构laminin-binding adhesin从链球菌在2.5 agalactiae,确定吗?决议。同源性,Lmb放入集群9家庭的ABC(磷酸腺苷磁带)交通系统。Lmb ABC-type相似solute-binding蛋白(sbp),其中两个相关球状结构域相互作用彼此形成一个metal-binding腔接口。由三个组氨酸和四面体地协调谷氨酸从两个领域。Lmb实验室与其他金属转运蛋白显示一个有趣的二聚作用的特性晶体中的分子不对称单位在所有的锌结合转运蛋白。Lmb的比较与锌结合ZnuA大肠杆菌和6803集胞藻属Lmb可能经历一个独特的结构重新排列在金属绑定和释放。Lmb的晶体结构提供了动力进一步调查分子基础层粘连蛋白绑定的人类病原体。无处不在的B组的血清型链球菌(GBS), Lmb可能的结构直接的发展一个有效的疫苗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号