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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites
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Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites

机译：Nucleant-mediated蛋白质结晶的应用微孔合成沸石

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摘要

Protein crystallization is still a major bottleneck in structural biology. As the current methodology of protein crystallization is a type of screening, it is usually difficult to crystallize important target proteins. It was thought that hetero-epitaxic growth from the surface of a mineral crystal acting as a nucleant would be an effective enhancer of protein crystallization. However, in spite of almost two decades of effort, a generally applicable hetero-epitaxic nucleant for protein crystallization has yet to be found. Here we introduce the first candidate for a universal hetero-epitaxic nucleant, microporous zeolite: a synthetic aluminosilicate crystalline polymer with regular micropores. It promotes a form-selective crystal nucleation of proteins and acts as a crystallization catalyst. The most successful zeolite nucleant was molecular sieve type 5A with a pore size of 5 angstrom and with bound Ca2+ ions. The zeolite-mediated crystallization improved the crystal quality in five out of six proteins tested. It provided new crystal forms with better resolution in two cases, larger crystals in one case, and zeolite-dependent crystal formations in two cases. The hetero-epitaxic growth of the zeolite-mediated crystals was confirmed by a crystal-packing analysis which revealed a layer-like structure in the crystal lattice.
机译：蛋白质结晶仍然是主要的在结构生物学的瓶颈。方法论的蛋白质结晶是一个类型筛选,它通常是困难的蛋白质结晶的重要目标。认为hetero-epitaxic增长的矿物晶体表面形成核的作用将是一个有效的增强剂的蛋白质结晶。几十年的努力,一个普遍适用的hetero-epitaxic形成核的蛋白质结晶尚未被发现。介绍第一个候选人普遍hetero-epitaxic形成核的微孔沸石:合成铝矽酸盐结晶聚合物与常规的微孔隙。蛋白质和form-selective晶体成核作为一个结晶催化剂。成功的沸石分子筛形成核的5类型的孔隙大小5埃绑定Ca2 +离子。结晶的晶体质量的改善五,六蛋白质检测。晶体形式两个更高的分辨率情况下,更大的晶体在一个案例中,和zeolite-dependent晶体结构在两个用例。zeolite-mediated晶体被证实了crystal-packing分析显示在晶格层结构。

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