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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Direct interaction between a human digestive protease and the mucoadhesive poly(acrylic acid).
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Direct interaction between a human digestive protease and the mucoadhesive poly(acrylic acid).

机译：人类的消化系统之间的直接交互蛋白酶和mucoadhesive聚(丙烯酸)。

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摘要

Carboxypeptidase A1 has been the subject of extensive research in the last 30 y and is one of the most widely studied zinc metalloenzymes. However, the three-dimensional structure of the human form of the enzyme is not yet available. This report describes the three-dimensional structure of human carboxypeptidase A1 (hCPA1) derived from crystals that belong to the tetragonal space group P4(3)2(1)2 and diffract to 1.6 A resolution. A description of the ternary complex hCPA1-Zn(2+)-poly(acrylic acid) is included as a model of the interaction of mucoadhesive polymers with proteases in the gastrointestinal tract. The direct mode of interaction between poly(acrylic acid) and the active site of the target protease was confirmed by in vitro inhibition assays. The structure was further analyzed in silico through the optimal docking-area method. The characterization of binding sites on the surface of hCPA1 and a comparison with other available carboxypeptidase structures provided further insights into the formation of multiprotein complexes and the activation mechanisms of carboxypeptidase zymogens. The high-resolution structure of hCPA1 provides an excellent template for the modelling of physiologically relevant carboxypeptidases and could also contribute to the design of specific agents for biomedical purposes.
机译：羧肽酶A1的主题广泛的研究在过去的30 y就是其中之一最广泛的研究近年锌。然而,三维的结构人类形体的酶还没有可用的。这份报告描述了三维结构的人类羧肽酶A1 (hCPA1)来自属于晶体正方空间群P4(3) 2(1) 2和衍射1.6一项决议。复杂的hCPA1-Zn(2 +)聚(丙烯酸)作为一个模型之间的相互作用与蛋白酶mucoadhesive聚合物胃肠道。聚(丙烯酸)和之间的相互作用目标蛋白酶的活性部位是证实通过体外抑制试验。进一步分析了硅片通过最优泊船区方法。表面结合位点hCPA1和比较与其他可用的羧肽酶结构提供了进一步的见解multiprotein复合物的形成羧肽酶的激活机制发酵菌。提供了一个优秀的造型的模板的生理相关的羧肽酶和也可以为特定的设计吗代理生物医学用途。

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