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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Pseudo-merohedral twinning in crystals of the dihaem c-type cytochrome DHC2 from Geobacter sulfurreducens.
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Pseudo-merohedral twinning in crystals of the dihaem c-type cytochrome DHC2 from Geobacter sulfurreducens.

机译：Pseudo-merohedral双晶的晶体dihaem c -型细胞色素DHC2核废料旁边sulfurreducens。

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摘要

The dihaem cytochrome DHC2 from Geobacter sulfurreducens has been described as a model system for the study of one of the conserved haem-packing motifs typically observed in multihaem cytochromes c, in which the covalent attachment of haem to the protein allows effective spatial clustering of redox centres and a high haem:protein ratio. Heterologously expressed DHC2 has been crystallized previously and the structure has been solved by multiple-wavelength anomalous dispersion (MAD) and refined to a resolution of 1.5 A (PDB code 2czs). Crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 39.65, b = 55.68, c = 39.64 A, beta = 105.91 degrees . Close inspection of the data indicated the presence of pseudo-merohedral twinning; while not hindering structure solution by MAD, this resulted in high refinement R factors. A re-assessment of the diffraction data for DHC2 is presented, together with a refined structure that includes the twinning present with a twinning fraction alpha of 0.32.
机译：dihaem细胞色素DHC2核废料旁边sulfurreducens被描述为一个模型研究系统的守恒的haem-packing图案通常观察到multihaem细胞色素c,共价附件的血红素蛋白质有效的氧化还原中心和空间聚类高血红素:蛋白质比例。表示DHC2结晶之前和结构已经被解决了多波长反常色散(疯了)和1.5精制的决议(PDB代码2 cz)。集团P2(1),晶胞参数= 39.65,b = 55.68, c = 39.64,β= 105.91度。仔细观察的数据显示pseudo-merohedral双晶的存在;阻碍结构解决方案,疯了,这个导致高细化R因素。再对DHC2的衍射数据,加上精致的结构包括在场的孪生双生分数α为0.32。

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