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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >High-molecular-weight polymers for protein crystallization: poly-gamma-glutamic acid-based precipitants.
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High-molecular-weight polymers for protein crystallization: poly-gamma-glutamic acid-based precipitants.

机译：高分子量聚合物对蛋白质结晶:poly-gamma-glutamic酸碱度沉淀剂。

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摘要

Protein crystallization has been revolutionized by the introduction of high-throughput technologies, which have led to a speeding up of the process while simultaneously reducing the amount of protein sample necessary. Nonetheless, the chemistry dimension of protein crystallization has remained relatively undeveloped. Most crystallization screens are based on the same set of precipitants. To address this shortcoming, the development of new protein precipitants based on poly-gamma-glutamic acid (PGA) polymers with different molecular-weight ranges is reported here: PGA-LM (low molecular weight) of approximately 400 kDa and PGA-HM (high molecular weight) of >1000 kDa. It is also demonstrated that protein precipitants can be expanded further to polymers with much higher molecular weight than those that are currently in use. Furthermore, the modification of PGA-like polymers by covalent attachments of glucosamine substantially improved their solubility without affecting their crystallization properties. Some preliminary PGA-based screens are presented here.
机译：蛋白质结晶已经彻底改变了高通量技术的引入,导致一个加速的过程同时减少的数量蛋白质样品的必要。化学方面的蛋白质结晶仍相对不发达。结晶屏幕是基于同一组沉淀剂。开发新的蛋白质沉淀剂的基础上poly-gamma-glutamic酸(PGA)聚合物不同分子量范围的报道: PGA-LM(低分子量)大约400 kDa PGA-HM(高分子> 1000 kDa的重量)。蛋白质沉淀剂可以进一步扩大与更高分子量的聚合物比那些目前正在使用。此外,PGA-like的修改聚合物共价附件的氨基葡萄糖大大提高了溶解度影响他们的结晶特性。这里给出初步PGA-based屏幕。

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