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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Meso-substituted cationic porphyrins interact with dsDNA and exhibit different localization patterns in radiation-induced fibrosarcoma cells.
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Meso-substituted cationic porphyrins interact with dsDNA and exhibit different localization patterns in radiation-induced fibrosarcoma cells.

机译：介孔取代的阳离子卟啉与dsDNA相互作用，并在辐射诱导的纤维肉瘤细胞中表现出不同的定位模式。

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摘要

Meso-substituted cationic porphyrins were examined for binding dsDNA. Subcellular localization time studies used Confocal Laser Scanning Microscopy of radiation-induced fibrosarcoma (RIF) cells incubated with porphyrins. Binding studies revealed a reversible interaction between porphyrin and dsDNA that is a function of DNA shape. Binding was inhibited at high salt concentrations, and enhanced by heat and DNA denaturants such as dimethyl formamide (DMF). Trans dicationic porphyrin required more stringent binding conditions than cis dicationic and tetracationic porphyrins. Phenol extraction of porphyrin from the DNA-porphyrin complex demonstrates that cationic porphyrins do not damage dsDNA at high concentrations. Localization studies within a 24-hour range reveal different distribution patterns. Metal chelates of tetracationic porphyrin exhibited a cytoplasmic localization with the exception of the zinc chelate. Localization of other metal chelates appears to be redistributed to lysosomes and mitochondria between 3 and 6 hours post-incubation. HPPH used in PDT clinical trials localizes to the cytoplasmic compartment.
机译：检查介孔取代的阳离子卟啉的结合dsDNA。亚细胞定位时间研究使用共聚焦激光扫描显微镜观察与卟啉一起孵育的辐射诱导的纤维肉瘤（RIF）细胞。结合研究揭示了卟啉和dsDNA之间可逆的相互作用，这是DNA形状的函数。在高盐浓度下抑制结合，并通过热和DNA变性剂（例如二甲基甲酰胺（DMF））增强结合。反式卟啉比顺式和四阳离子卟啉需要更严格的结合条件。从DNA-卟啉复合物中苯酚提取卟啉表明，阳离子卟啉在高浓度下不会破坏dsDNA。 24小时范围内的本地化研究显示了不同的分布模式。四价卟啉的金属螯合物除锌螯合物外，还表现出胞质定位。在孵育后3到6个小时之间，其他金属螯合物的定位似乎重新分配到了溶酶体和线粒体。 PDT临床试验中使用的HPPH定位于细胞质区室。

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