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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >EMatch: an efficient method for aligning atomic resolution subunits into intermediate-resolution cryo-EM maps of large macromolecular assemblies
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EMatch: an efficient method for aligning atomic resolution subunits into intermediate-resolution cryo-EM maps of large macromolecular assemblies

机译：调整原子EMatch:一种有效的方法分辨率的子单元到intermediate-resolution低温电子显微镜的地图大大分子组装

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摘要

Structural analysis of biological machines is essential for inferring their function and mechanism. Nevertheless, owing to their large size and instability, deciphering the atomic structure of macromolecular assemblies is still considered as a challenging task that cannot keep up with the rapid advances in the protein- identification process. In contrast, structural data at lower resolution is becoming more and more available owing to recent advances in cryo- electron microscopy (cryoEM) techniques. Once a cryo- EM map is acquired, one of the basic questions asked is what are the folds of the components in the assembly and what is their configuration. Here, a novel knowledge- based computational method, named EMatch, towards tackling this task for cryo- EM maps at 6-10 angstrom resolution is presented. The method recognizes and locates possible atomic resolution structural homologues of protein domains in the assembly. The strengths of EMatch are demonstrated on a cryo- EM map of native GroEL at 6 angstrom resolution.
机译：结构分析的生物机器基本功能和推断机制。大小和不稳定,破译原子大分子的结构组件仍在认为是一个具有挑战性的任务,不能保持蛋白质——的快速进步识别过程。低分辨率数据变得越来越更由于在低温-最新进展电子显微镜(cryoEM)技术。低温- EM地图是后天获得的,基本的国家之一问题是折叠的组件在组装和他们是什么配置。计算方法,名叫EMatch,低温处理这个任务——EM地图在6 - 10埃分辨率。识别和定位可能的原子分辨率结构蛋白质域的同系物组装。证明在低温- EM的原生GroEL地图6埃分辨率。

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