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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >So how do you know you have a macromolecular complex?
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So how do you know you have a macromolecular complex?

机译：所以你怎么知道你有一个大分子复杂吗?

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摘要

Protein in crystal form is at an extremely high concentration and yet retains the complex secondary structure that defines an active protein. The protein crystal itself is made up of a repeating lattice of protein-protein and protein-solvent interactions. The problem that confronts any crystallographer is to identify those interactions that represent physiological interactions and those that do not. This review explores the tools that are available to provide such information using the original crystal liquor as a sample. The review is aimed at postgraduate and postdoctoral researchers who may well be coming up against this problem for the first time. Techniques are discussed that will provide information on the stoichiometry of complexes as well as low-resolution information on complex structure. Together, these data will help to identify the physiological complex.
机译：蛋白质晶体形式是极高的浓度,但保留了复杂二级结构,定义了一个活跃蛋白质。一个重复的蛋白质晶格protein-solvent交互。面对任何晶体是识别那些代表生理的交互交互和那些不。探讨了工具可用来提供这些信息用最初的晶体酒作为一个样本。研究生和博士后研究人员是针对这个问题的出现已经不是第一次了。提供信息的化学计量学复合物以及低分辨率信息在复杂的结构。帮助识别生理复杂。

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