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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The first structure of a cold-active catalase from Vibrio salmonicida at 1.96 angstrom reveals structural aspects of cold adaptation
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The first structure of a cold-active catalase from Vibrio salmonicida at 1.96 angstrom reveals structural aspects of cold adaptation

机译：第一个cold-active过氧化氢酶的结构弧菌salmonicida为1.96埃冷适应的结构方面

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摘要

The cold-adapted catalase from the fish- pathogenic bacterium Vibrio salmonicida (VSC) has recently been characterized and shown to be two times more catalytically efficient compared with catalase from the mesophilic human pathogen Proteus mirabilis [PMC; Lorentzen et al. (2006), Extremophiles, 10, 427-440]. VSC is also less temperature-stable, with a half-life of 5 min at 333 K compared with 50 min for PMC. This was the background for solving the crystal structure of the cold-adapted VSC to 1.96 angstrom and performing an extensive structural comparison of VSC and PMC. The comparison revealed that the entrance ( the major channel) leading to the catalytically essential haem group, is locally more flexible and slightly wider in VSC. This might explain the enhanced catalytic efficiency of the nearly diffusion-controlled degradation of hydrogen peroxide into water and molecular oxygen in VSC. The reduced thermal stability of the cold-adapted VSC may be explained by a reduced number of ion-pair networks. The four C-terminal alpha-helices are displaced in the structures, probably owing to missing ionic interactions in VSC compared with PMC, and this is postulated as an initiation site for unfolding the cold-adapted enzyme. VSC is the first crystal structure reported of a cold-adapted monofunctional haem-containing catalase.
机译：冷鱼-过氧化氢酶细菌致病性弧菌salmonicida (VSC)最近两个特征列示倍相比,催化地有效嗜中温人类病原体的过氧化氢酶海神mirabilis [PMC;极端微生物,427 - 440]。temperature-stable,半衰期为5分钟333 K与PMC 50分钟。解决的晶体结构的背景冷VSC 1.96埃,执行一个广泛的结构的比较VSC和PMC。入口(主要通道)导致的催化地必不可少的血红素组,是本地的更加灵活和VSC略宽。或许可以解释增强的催化效率近diffusion-controlled退化的过氧化氢转化为水和氧气VSC。冷VSC可能解释为减少离子对网络的数量。阿尔法螺旋中流离失所的结构,可能由于缺少离子相互作用VSC与PMC相比,这是假定展开冷的起始点酶。的低温单功能的报道haem-containing过氧化氢酶。

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