Cryogenic ( 20 K) helium cooling mitigates radiation damage to protein crystals

Chinte U; Shah B; Chen YSPinkerton AAConstance ASHanson BL

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Cryogenic ( 20 K) helium cooling mitigates radiation damage to protein crystals

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Cryogenic ( 20 K) helium cooling mitigates radiation damage to protein crystals

机译：低温( 20 K)氦冷却了辐射损伤蛋白质晶体

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In experiments conducted at the Bio-CARS beamline 14-BM-C (APS, Argonne National Laboratory, USA), Streptomyces rubiginosus D-xylose isomerase (EC 5.3.1.5) crystals were used to test the effect of cryogen temperature on radiation damage. Crystals cooled using a helium cryostat at an 8 K set temperature consistently showed less decay in the signal-to-noise ratio, , and in average intensity, , compared with those cooled with a nitrogen cryostat set to 100 K. Multiple crystals grown using ammonium sulfate as precipitant were used at each cryostat set temperature and comparisons were made for crystals of similar size and diffraction resolution. Maximum resolution for the crystals was 1.1 - 1.3 angstrom, with He at 25% compared with crystals cooled with N-2 at 100 K.

机译：在实验Bio-CARS beamline美国阿贡国家实验室14-BM-C (APS),链霉菌属rubiginosus D-xylose异构酶(EC5.3.1.5)晶体被用来测试的影响致冷剂温度对辐射损伤。使用氦冷却低温恒温器在一组8 K温度持续衰减较少信噪比,,和平均强度,,相比冷却与氮低温恒温器设置为100 K。多个使用硫酸铵晶体生长沉淀剂被使用在每一个低温恒温器设置温度和比较了相似的晶体尺寸和衍射决议。1.1 - 1.3埃,他在 25%的数据相比,晶体冷却n - 100 K。

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《Acta crystallographica.Section D. Biological crystallography》 |2007年第4期|486-492|共7页
作者
Chinte U; Shah B; Chen YSPinkerton AAConstance ASHanson BL;
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作者单位

Argonne Natl Lab, Bio CARS, Adv Photon Source, Argonne, IL 60439 USA;

Univ Toledo, Dept Chem & Environm Engn, Toledo, OH 43606 USA;

Univ Toledo, Dept Chem, Toledo, OH 43606 USA;

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正文语种英语
中图分类化学;
关键词
Hydrogen; molecules; RadicalsCrystallographycrystalsX-ray diffractionsynchrotron radiationheliumCryostatsRadiation InjuriesON-SET TEMPERATURE;

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Cryogenic ( 20 K) helium cooling mitigates radiation damage to protein crystals

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