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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin
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Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin

机译：结构研究花生凝集素包裹着双糖涉及不同的联系:进一步的洞察和结构凝集素的相互作用

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摘要

Crystal structures of peanut lectin complexed with Gal beta 1-3Gal, methyl-T-antigen, Gal beta 1-6GalNAc, Gal alpha 1-3Gal and Gal alpha 1-6Glc and that of a crystal grown in the presence of Gal alpha 1-3 Gal beta 1-4Gal have been determined using data collected at 100 K. The use of water bridges as a strategy for generating carbohydrate specificity was previously deduced from the complexes of the lectin with lactose (Gal beta 1-4Glc) and T-antigen (Gal beta 1-3GalNAc). This has been confirmed by the analysis of the complexes with Gal beta 1-3Gal and methyl-T-antigen (Gal beta 1-3GalNAc-alpha-OMe). A detailed analysis of lectin-sugar interactions in the complexes shows that they are more extensive when the beta-anomer is involved in the linkage. As expected, the second sugar residue is ill-defined when the linkage is 1 -> 6. There are more than two dozen water molecules which occur in the hydration shells of all structures determined at resolutions better than 2.5 angstrom. Most of them are involved in stabilizing the structure, particularly loops. Water molecules involved in lectin-sugar interactions are also substantially conserved. The lectin molecule is fairly rigid and does not appear to be affected by changes in temperature.
机译：晶体结构的花生凝集素包裹着加β1-3Gal methyl-T-antigen,加β1-6GalNAc,加α1-3Gal和加α1-6Glc和晶体生长在的存在加α1 - 3加β1-4Gal决定使用收集的数据在100 K。桥梁作为生成的策略碳水化合物的特异性之前推断与乳糖凝集素的复合物(加β1-4Glc)和t抗原(加β1-3GalNAc)。的分析与加β1-3Gal复合物和methyl-T-antigen(加β1-3GalNAc-alpha-OMe)。复合物中的lectin-sugar交互显示beta-anomer时他们更广泛是联系在一起的。第二个糖残基是模糊的连杆是1 - > 6。水分子发生水合作用壳的结构决定比2.5埃分辨率。他们参与稳定结构,尤其是循环。lectin-sugar交互作用也显著守恒的。似乎,不受变化的影响温度。

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