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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc.
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Structural basis for the carbohydrate-specificity of basic winged-bean lectin and its differential affinity for Gal and GalNAc.

机译：carbohydrate-specificity结构基础基本的棱豆凝集素及其微分亲和力加和GalNAc。

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摘要

The crystal structure of the complexes of basic winged-bean lectin with galactose, 2-methoxygalactose, N-acetylgalactosamine and methyl-alpha-N-acetylgalactosamine have been determined. Lectin-sugar interactions involve four hydrogen bonds and a stacking interaction in all of the complexes. In addition, an N-H...O hydrogen bond involving the hydroxyl group at C2 exists in the galactose and 2-methoxygalactose complexes. An additional hydrophobic interaction involving the methyl group in the latter leads to the higher affinity of the methyl derivative. In the lectin-N-acetylgalactosamine complex the N-H...O hydrogen bond is lost, but a compensatory hydrogen bond is formed involving the O atom of the acetamido group. In addition, the CH(3) moiety of the acetamido group is involved in hydrophobic interactions. Consequently, the 2-methyl and acetamido derivatives of galactose have nearly the same affinity for the lectin. The methyl group alpha-linked to the galactose takes part in additional hydrophobic interactions. Therefore, methyl-alpha-N-acetylgalactosamine has a higher affinity than N-acetylgalactosamine for the lectin. The structures of basic winged-bean lectin-sugar complexes provide a framework for examining the relative affinity of galactose and galactosamine for the lectins that bind to them. The complexes also lead to a structural explanation for the blood-group specificity of basic winged-bean lectin.
机译：配合物的晶体结构的基础棱豆与半乳糖凝集素,2-methoxygalactose N-acetylgalactosamine和methyl-alpha-N-acetylgalactosamine一直确定。四个氢键和堆积相互作用所有的复合物。涉及的羟基氢键C2存在于半乳糖和2-methoxygalactose复合物。涉及后者导致的甲基高亲和力的甲基衍生物。lectin-N-acetylgalactosamine复杂的- h……涉及O原子形成氢键acetamido组。一半acetamido集团的参与疏水相互作用。2 -甲基和半乳糖的acetamido衍生品几乎相同的凝集素亲和。甲基alpha-linked的半乳糖参加额外的疏水相互作用。因此,methyl-alpha-N-acetylgalactosamine亲和力高于N-acetylgalactosamine凝集素。lectin-sugar复合物提供了一个框架研究半乳糖的相对亲和力和氨基半乳糖凝集素的结合。复合物也导致结构性的解释的血型专一性基本的棱豆凝集素。

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