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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >A survey of protein-protein complex crystallizations.
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A survey of protein-protein complex crystallizations.

机译：蛋白质复杂的调查结晶方法。

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A survey of crystallization conditions was carried out for 650 published protein-protein complexes in the Protein Data Bank (PDB) of the Research Collaboratory for Structural Bioinformatics (RCSB). This resulted in the establishment of a Protein Complex Crystallization Database (PCCD) and a set of configuration-space boundaries for protein-complex crystallizations. Overall, polyethylene glycol (PEG) based conditions accounted for 70-80% of all crystallizations, with PEG 3000-4000, 5000-6000 and 8000 being the most frequently used. The median values of PEG concentrations were between 10 and 20% and were inversely correlated with their molecular weights. Ammonium sulfate remained the most favorable salt precipitant, with a median concentration of 1.6 M. The crystallization pH for the vast majority of protein complexes was between 5.0 and 8.0. Overall, the boundaries for the crystallization configuration space of protein complexes appear to be more restricted than those of soluble proteins. This may reflect the limited stability and solubility of protein-protein complexes. Based on statistical analysis of the database, a sparse-matrix and a systematic buffer and pH screen were formulated to best represent the crystallization of protein complexes.
机译：结晶条件进行的调查650年出版的蛋白质复合物蛋白质数据库(PDB)的研究合作实验室结构生物信息学(RCSB)。蛋白复合物结晶数据库(PCCD)和一组配置空间的界限蛋白复合物的结晶方法。聚乙二醇(PEG)的基础条件占70 - 80%的结晶方法,与PEG 3000 - 4000、5000 - 6000和8000的最经常使用的。10至20%,浓度负相关的分子权重。有利的盐沉淀剂,值浓度为1.6 m .结晶的pH值对于绝大多数的蛋白复合物在5.0和8.0之间。的结晶配置空间蛋白复合物似乎更受限制比可溶性蛋白质。有限的稳定和溶解度蛋白质复合物。分析数据库的一个稀疏矩阵和一个系统的缓冲和pH值屏幕被制定最好的代表蛋白质的结晶复合物。

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