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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Development of an automated large-scale protein-crystallization and monitoring system for high-throughput protein-structure analyses
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Development of an automated large-scale protein-crystallization and monitoring system for high-throughput protein-structure analyses

机译：一个自动化的大规模的发展蛋白质晶体和监控系统高通量分析工作

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摘要

Protein crystallization remains one of the bottlenecks in crystallographic analysis of macromolecules. An automated large-scale protein-crystallization system named PXS has been developed consisting of the following subsystems, which proceed in parallel under unified control software: dispensing precipitants and protein solutions, sealing crystallization plates, carrying robot, incubators, observation system and image-storage server. A sitting-drop crystallization plate specialized for PXS has also been designed and developed. PXS can set up 7680 drops for vapour diffusion per hour, which includes time for replenishing supplies such as disposable tips and crystallization plates. Images of the crystallization drops are automatically recorded according to a preprogrammed schedule and can be viewed by users remotely using web-based browser software. A number of protein crystals were successfully produced and several protein structures could be determined directly from crystals grown by PXS. In other cases, X-ray quality crystals were obtained by further optimization by manual screening based on the conditions found by PXS.
机译：蛋白质结晶仍是其中一个瓶颈在晶体的分析大分子。蛋白质晶体系统不用再被命名开发包括以下子系统,并行进行统一控制软件:沉淀剂和蛋白质解决方案,密封结晶板,带着机器人,孵化器,观测系统和图像存储服务器。结晶板专门用于不用设计和开发。每小时7680下降为蒸汽扩散,包括时间补充供应等一次性的技巧和结晶盘子。结晶时的图像根据一个自动记录预排程序的安排,可以被用户远程使用网络浏览器软件。成功的蛋白质晶体数量生产和几个蛋白质结构直接从晶体增长了不用再决定。在其他情况下,x射线晶体质量通过进一步优化手册根据条件筛选发现不用。

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