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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Preliminary analysis of crystals of panicum mosaic virus (PMV) by X-ray diffraction and atomic force microscopy
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Preliminary analysis of crystals of panicum mosaic virus (PMV) by X-ray diffraction and atomic force microscopy

机译：初步分析晶体的黍马赛克病毒(PMV)通过x射线衍射、原子力显微镜

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摘要

Panicum mosaic virus (PMV), a spherical virus of diameter about 300 Angstrom, has been crystallized in a form suitable for high-resolution structural analysis. The crystals were grown from 15% PEG 400 at room temperature and could be flash-frozen directly from their mother liquor. The crystals diffracted to beyond 2.7 Angstrom resolution. A data set was collected at 100 K to an effective resolution of 3.2 Angstrom [Weiss (2001), J. Appl. Cryst. 34, 130-135]. The crystals belonged to space group P2(1), with unit-cell parameters a = 411.7, b = 403.9, c = 412.5 Angstrom, beta = 89.7degrees. Self-rotation functions and molecular replacement with tobacco necrosis virus as the probe model yielded tentative positions and orientations for the two entire virus particles comprising the asymmetric unit and implied a pseudo-face-centered cubic packing arrangement. Investigation of lightly glutaraldehyde-fixed crystals in water using atomic force microscopy confirms the packing arrangement given by the molecular-replacement result. The images also show that contaminating virions of the satellite virus to PMV, known as satellite panicum mosaic virus (SPMV), can be incorporated into the PMV crystals by insertion into the interstices between PMV virions in the lattice. This is the first observation of such a phenomenon in macromolecular crystals.
机译：黍花叶病毒(PMV),球形病毒直径约300埃结晶形式适合高分辨率结构分析。从15% PEG 400在室温下,可以直接从他们被迅速冻结母液。2.7埃分辨率。3.2在100 K的有效决议埃维斯(2001),j .达成。130 - 135年)。P2(1),晶胞参数= 411.7,=403.9, c = 412.5埃,β= 89.7度。固有转动功能和分子置换与烟草坏死病毒探测模型取得了初步的位置和方向两个完整的病毒粒子组成不对称单元和暗示pseudo-face-centered立方包装安排。调查轻轻glutaraldehyde-fixed晶体在水中使用原子力显微镜确认的包装安排molecular-replacement结果。表明污染病毒粒子的卫星病毒PMV,称为卫星黍马赛克病毒(SPMV),可以纳入结构晶体插入间隙PMV病毒粒子之间的晶格。首先观察的现象大分子晶体。

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