Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine.

Koepke J; Ma X; Fritzsch GMichel HStockigt J

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine.

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Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine.

机译：结晶和初步的x射线分析strictosidine合成酶及其复杂的衬底色胺。

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Strictosidine synthase (STR1) is a central enzyme that participates in the biosynthesis of almost all plant monoterpenoid indole alkaloids. After heterologous expression in Escherichia coli, crystals of STR1 and its substrate complex with tryptamine were obtained by the hanging-drop technique at 302-304 K with potassium sodium tartrate tetrahydrate as precipitant. All crystals belong to space group R3. The native STR1 crystals diffract to 2.95 A and have unit-cell parameters a = b = 150.3, c = 122.4 A. The tryptamine complex crystals diffract to 2.38 A, with unit-cell parameters a = b = 147.3, c = 122.3 A.

机译：Strictosidine合成酶(STR1)是一个中央酶参与的生物合成所有植物monoterpenoid吲哚生物碱。不同的表达在大肠杆菌中,晶体STR1及其基质复杂色胺得到的悬滴技术与钠钾302 - 304 K酒石酸四水合物作为沉淀剂。水晶属于空间群R3。STR1晶体衍射,2.95晶胞参数a = b = 150.3, c = 122.4。色胺复杂晶体衍射,至2.38与晶胞参数,A = b = 147.3, c =122.3。

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《Acta crystallographica.Section D. Biological crystallography》 |2005年第6期|690-693|共4页
作者
Koepke J; Ma X; Fritzsch GMichel HStockigt J;
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作者单位

Department of Molecular Membrane Biology, Max-Planck-Institute of Biophysics, Marie-Curie-Strasse 15, D-60439 Frankfurt/Main, Germany.;

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正文语种英语
中图分类化学;
关键词
strictosidine synthetase; sodium potassium tartrate; TryptaminesX-ray analysisX-raysSecologanin Tryptamine AlkaloidsstrictosidineMMP3 geneCrystallization;

机译：strictosidine合成酶;钾钠酒石酸;TryptaminesX-ray;

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Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine.

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