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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >A modified vapor-diffusion crystallization protocol that uses a common dehydrating agent
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A modified vapor-diffusion crystallization protocol that uses a common dehydrating agent

机译：修改后的扩散结晶协议,它使用一种常见的脱水剂

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摘要

In the vapor-diffusion protein-crystallization method, a small drop containing protein sample mixed with a crystallization solution is equilibrated against a reservoir solution in a sealed chamber. Whereas the chemical composition of the crystallization solution is critical for success, the primary role of the reservoir solution is to slowly concentrate the crystallization drop in a controlled fashion. Accordingly, it might be possible to use any reservoir solution of appropriate dehydrating strength. The important practical consequence is that many different experiments can share the same reservoir solution. This approach, called the 'shared reservoir solution' method, significantly simplifies manual and robotic experiment setup, reduces cost and allows a completely new design of optically superior and higher density crystallization plates. Although this research was motivated by these practical advantages, recent reports and the authors' results indicate that this method may actually increase crystallization success. The authors suggest that this may indicate that a protein has a preferred water activity for crystallization. Here, present practical and theoretical considerations as well as experimental tests of the shared reservoir solution method are presented.
机译：扩散的蛋白质晶体方法,包含蛋白质样品的小滴与结晶溶液混合在一个平衡对储层的解决方案密封室。结晶的解决方案是至关重要的成功,水库的主要作用解决方法是慢慢地集中注意力结晶控制方式。因此,有可能使用任何储层解决方案适当的脱水的力量。许多不同的实验可以共享同一储层的解决方案。共享储层解决方案的方法,大大简化了人工和机器人实验设置,减少成本和允许全新的光学设计和优越高密度结晶盘子。这项研究是出于这些实际优势,最近的报告和作者的结果表明,这种方法实际上可能增加成功结晶。认为这可能表明一种蛋白质一个首选水结晶。在这里,现在的实践和理论注意事项以及实验测试共享储层解决方案方法提出了。

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