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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Radiation-induced site-specific damage of mercury derivatives: phasing and implications.
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Radiation-induced site-specific damage of mercury derivatives: phasing and implications.

机译：辐射诱导特定场地损坏的水银衍生品:分阶段和影响。

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摘要

The behavior of mercury-derivatized triclinic crystals of a 60 kDa protein target from the New York Structural GenomiX Research Consortium provides novel insights into the mechanism of heavy-atom-specific radiation damage and its potential exploitation for de novo structure solution. Despite significant anomalous signal, structure solution by classic SAD and MAD phasing approaches was not successful. A detailed analysis revealed that significant isomorphic variation of the diffracted intensities was induced by X-ray irradiation. These intensity changes allowed the crystal structure to be solved by the radiation-damage-induced phasing (RIP) technique. Inspection of the crystal structure and electron-density maps demonstrated that the covalent S-Hg bonds at all four derivatized cysteine sites were much more susceptible to radiation-induced cleavage than other bonds typically present in native proteins. A simple diagnostic is described to identify the fingerprint of such decay at the time of data collection/processing. The rapid radiation-induced decomposition of mercury adducts is consistent with the difficulties frequently associated with the experimental phasing of mercury derivatives and suggests a straightforward solution to overcome this limitation by radiation-damage-induced phasing with anomalous scattering (RIPAS). These results indicate that historically recalcitrant and newly emerging difficulties associated with Hg phasing should be revisited.
机译：的行为mercury-derivatized三斜晶系的晶体的60 kDa蛋白质的新目标纽约结构性GenomiX研究财团为的机理提供了新的见解heavy-atom-specific辐射损伤及其新创的潜在开发结构解决方案。结构的解决方案,经典的悲伤和疯狂的定相方法是不成功的。分析显示,重大的同构衍射强度的变化由x射线辐照。使晶体结构变化通过radiation-damage-induced逐步解决(RIP)技术。结构和电子密度地图了在所有四个共价S-Hg债券derivatized半胱氨酸站点得多容易受到辐射诱导乳沟其他债券通常出现在本地的蛋白质。一个简单的诊断识别描述指纹的衰变时的数据收集和处理。辐射诱导分解的汞加合物与困难是一致的经常与实验有关定相汞的衍生品和建议为了克服这个简单的解决方案限制通过radiation-damage-induced逐步与反常散射(里帕)。表明历史上顽固的和新新兴的困难与Hg定相关联应该重新审视。

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