The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79.

Mavrodi DV; Bleimling N; Thomashow LSBlankenfeldt W

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79.

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The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79.

机译：净化、结晶和初步的PhzF结构表征,一种关键酶在phenazine-biosynthesis通路荧光假2 - 79。

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Phenazines produced by members of several bacterial genera are biologically active metabolites that function in microbial competitiveness, the suppression of soil-borne plant diseases and virulence in infectious disease. Despite recent progress towards understanding the biochemistry of phenazine synthesis, the key reactions leading to the formation of the heterocyclic scaffold common to all phenazine compounds remain obscure. Pseudomonas fluorescens 2-79 contains seven phenazine (phz) genes that encode components of the pathway for biosynthesis of phenazine-1-carboxylic acid. A central step in this pathway involves the condensation of two identical precursor molecules derived from chorismic acid and is catalysed by the product of the phzF gene. In this study, recombinant PhzF was purified and crystallized from PEG 4000/ammonium sulfate/sodium citrate pH 5.6. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 56.3, c = 156.4 A. They contain one monomer in the asymmetric unit and diffract to better than 1.7 A on synchrotron beamlines. Crystals of seleno-L-methionine-labelled PhzF have been obtained and SAD data are reported.

机译：吩嗪由几个成员细菌属生物活性功能微生物的代谢物竞争力,抑制土传植物在传染性疾病和毒性疾病。理解吩嗪的生物化学合成、反应导致的关键形成杂环支架常见的所有吩嗪化合物依然模糊。荧光假2 - 79包含七个吩嗪(phz)基因编码的组成部分生物合成的途径phenazine-1-carboxylic酸。这个途径包括两个的凝结来自相同的前体分子分支酸,是催化的产品phzF基因。纯化和结晶挂钩吗4000 /硫酸铵/柠檬酸钠pH值5.6。水晶属于空间群P3(1) 21或P3(2) 21日晶胞参数a = b = 56.3,c = 156.4。不对称单元和衍射比1.7更好在同步加速器beamlines。seleno-L-methionine-labelled PhzF一直获得和悲伤的数据报告。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第1期|184-186|共3页
作者
Mavrodi DV; Bleimling N; Thomashow LSBlankenfeldt W;
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Department of Plant Pathology, Washington State University, Pullman, Washington 99164-6430, USA.;

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正文语种英语
中图分类化学;
关键词
phenazines; Chorismic Acid; PurificationPseudomonas fluorescensAnabolismPathway;

机译：吩嗪;分支酸;PurificationPseudomonas;

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The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79.

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